In-cell covalent labeling of reactive His-tag fused proteins

Shohei Uchinomiya, Hiroshi Nonaka, Sho Wakayama, Akio Ojida, Itaru Hamachi

研究成果: ジャーナルへの寄稿記事

36 引用 (Scopus)

抄録

A new method for in-cell protein labeling was developed. This method employed a binding-induced nucleophilic reaction between the Cys-appended His-tag and the Ni(ii)-NTA containing an α-chloroacetamide. Using this method, not only labeling of His-tag fused proteins but also the detection of a protein-protein interaction was achieved inside living cells.

元の言語英語
ページ(範囲)5022-5024
ページ数3
ジャーナルChemical Communications
49
発行部数44
DOI
出版物ステータス出版済み - 6 4 2013

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Labeling
Proteins
Cells

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

これを引用

In-cell covalent labeling of reactive His-tag fused proteins. / Uchinomiya, Shohei; Nonaka, Hiroshi; Wakayama, Sho; Ojida, Akio; Hamachi, Itaru.

:: Chemical Communications, 巻 49, 番号 44, 04.06.2013, p. 5022-5024.

研究成果: ジャーナルへの寄稿記事

Uchinomiya, Shohei ; Nonaka, Hiroshi ; Wakayama, Sho ; Ojida, Akio ; Hamachi, Itaru. / In-cell covalent labeling of reactive His-tag fused proteins. :: Chemical Communications. 2013 ; 巻 49, 番号 44. pp. 5022-5024.
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