In ovo nanoinjection of triclosan, diclofenac and carbamazepine affects embryonic development of medaka fish (Oryzias latipes)

Mohamed Nassef, Sang Gyoon Kim, Masanori Seki, Ikjoon Kang, Takeshi Hano, Yohei Shimasaki, Yuji Oshima

研究成果: ジャーナルへの寄稿記事

55 引用 (Scopus)

抄録

We examined the toxicity of three pharmaceuticals and personal care products (PPCPs) - triclosan (TCS), diclofenac (DCF), and carbamazepine (CBMZ) - on embryonic development of Japanese medaka (Oryzias latipes) using in ovo nanoinjection. Medaka eggs (8 h post-fertilization; late blastula stage) were injected with 0.5 nL of triolein (vehicle control) or 0.5 nL of PPCPs, using different doses of TCS (1, 5, or 9 ng), DCF (1, 5, or 12 ng), or CBMZ (1, 5, or 12 ng) per egg in triolein, in addition to uninjected control. Following injection, we recorded survival, embryonic lesions, delay in embryonic development (eye, embryonic body and internal organs), heart beat rate, hatchability, and hatching time of embryos and upward swimming of larvae. As a result, injected PPCPs caused toxic responses to medaka embryos during embryonic development and around the day of hatching. Based on estimated EC50 values of PPCPs doses on survival of injected embryos at hatching, TCS (at a dose of 4.2 ng egg-1) was generally more toxic to medaka embryos, followed by DCF (6.0 ng egg-1), and CBMZ (13.1 ng egg-1). We conclude that the nanoinjection medaka embryos model is a valuable tool for analyzing the effects of chemicals on the development of fish embryos and feasibility of nanoinjecting PPCPs into small fish eggs perhaps mimicking early exposure resulting from oocyte uptake of contaminants from maternal extra gonadal tissues.

元の言語英語
ページ(範囲)966-973
ページ数8
ジャーナルChemosphere
79
発行部数9
DOI
出版物ステータス出版済み - 5 1 2010

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Triclosan
Oryzias
Diclofenac
Carbamazepine
embryonic development
Pharmaceutical Services
Drug products
Fish
Embryonic Development
embryo
Fishes
egg
Embryonic Structures
Ovum
Triolein
fish
Pharmaceutical Preparations
Poisons
hatching
Eggs

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Environmental Chemistry

これを引用

In ovo nanoinjection of triclosan, diclofenac and carbamazepine affects embryonic development of medaka fish (Oryzias latipes). / Nassef, Mohamed; Kim, Sang Gyoon; Seki, Masanori; Kang, Ikjoon; Hano, Takeshi; Shimasaki, Yohei; Oshima, Yuji.

:: Chemosphere, 巻 79, 番号 9, 01.05.2010, p. 966-973.

研究成果: ジャーナルへの寄稿記事

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abstract = "We examined the toxicity of three pharmaceuticals and personal care products (PPCPs) - triclosan (TCS), diclofenac (DCF), and carbamazepine (CBMZ) - on embryonic development of Japanese medaka (Oryzias latipes) using in ovo nanoinjection. Medaka eggs (8 h post-fertilization; late blastula stage) were injected with 0.5 nL of triolein (vehicle control) or 0.5 nL of PPCPs, using different doses of TCS (1, 5, or 9 ng), DCF (1, 5, or 12 ng), or CBMZ (1, 5, or 12 ng) per egg in triolein, in addition to uninjected control. Following injection, we recorded survival, embryonic lesions, delay in embryonic development (eye, embryonic body and internal organs), heart beat rate, hatchability, and hatching time of embryos and upward swimming of larvae. As a result, injected PPCPs caused toxic responses to medaka embryos during embryonic development and around the day of hatching. Based on estimated EC50 values of PPCPs doses on survival of injected embryos at hatching, TCS (at a dose of 4.2 ng egg-1) was generally more toxic to medaka embryos, followed by DCF (6.0 ng egg-1), and CBMZ (13.1 ng egg-1). We conclude that the nanoinjection medaka embryos model is a valuable tool for analyzing the effects of chemicals on the development of fish embryos and feasibility of nanoinjecting PPCPs into small fish eggs perhaps mimicking early exposure resulting from oocyte uptake of contaminants from maternal extra gonadal tissues.",
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AU - Nassef, Mohamed

AU - Kim, Sang Gyoon

AU - Seki, Masanori

AU - Kang, Ikjoon

AU - Hano, Takeshi

AU - Shimasaki, Yohei

AU - Oshima, Yuji

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N2 - We examined the toxicity of three pharmaceuticals and personal care products (PPCPs) - triclosan (TCS), diclofenac (DCF), and carbamazepine (CBMZ) - on embryonic development of Japanese medaka (Oryzias latipes) using in ovo nanoinjection. Medaka eggs (8 h post-fertilization; late blastula stage) were injected with 0.5 nL of triolein (vehicle control) or 0.5 nL of PPCPs, using different doses of TCS (1, 5, or 9 ng), DCF (1, 5, or 12 ng), or CBMZ (1, 5, or 12 ng) per egg in triolein, in addition to uninjected control. Following injection, we recorded survival, embryonic lesions, delay in embryonic development (eye, embryonic body and internal organs), heart beat rate, hatchability, and hatching time of embryos and upward swimming of larvae. As a result, injected PPCPs caused toxic responses to medaka embryos during embryonic development and around the day of hatching. Based on estimated EC50 values of PPCPs doses on survival of injected embryos at hatching, TCS (at a dose of 4.2 ng egg-1) was generally more toxic to medaka embryos, followed by DCF (6.0 ng egg-1), and CBMZ (13.1 ng egg-1). We conclude that the nanoinjection medaka embryos model is a valuable tool for analyzing the effects of chemicals on the development of fish embryos and feasibility of nanoinjecting PPCPs into small fish eggs perhaps mimicking early exposure resulting from oocyte uptake of contaminants from maternal extra gonadal tissues.

AB - We examined the toxicity of three pharmaceuticals and personal care products (PPCPs) - triclosan (TCS), diclofenac (DCF), and carbamazepine (CBMZ) - on embryonic development of Japanese medaka (Oryzias latipes) using in ovo nanoinjection. Medaka eggs (8 h post-fertilization; late blastula stage) were injected with 0.5 nL of triolein (vehicle control) or 0.5 nL of PPCPs, using different doses of TCS (1, 5, or 9 ng), DCF (1, 5, or 12 ng), or CBMZ (1, 5, or 12 ng) per egg in triolein, in addition to uninjected control. Following injection, we recorded survival, embryonic lesions, delay in embryonic development (eye, embryonic body and internal organs), heart beat rate, hatchability, and hatching time of embryos and upward swimming of larvae. As a result, injected PPCPs caused toxic responses to medaka embryos during embryonic development and around the day of hatching. Based on estimated EC50 values of PPCPs doses on survival of injected embryos at hatching, TCS (at a dose of 4.2 ng egg-1) was generally more toxic to medaka embryos, followed by DCF (6.0 ng egg-1), and CBMZ (13.1 ng egg-1). We conclude that the nanoinjection medaka embryos model is a valuable tool for analyzing the effects of chemicals on the development of fish embryos and feasibility of nanoinjecting PPCPs into small fish eggs perhaps mimicking early exposure resulting from oocyte uptake of contaminants from maternal extra gonadal tissues.

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