In vitro decidualization of rat endometrial stromal cells

Kenji Matsumoto, Nobuhiko Yamauchi, Ryo Watanabe, Shinji Oozono, Kaiyu Kubota, Kyohei Nishimura, Chris Wood, Tomoki Soh, Kei Ichirou Kizaki, Masa Aki Hattori

研究成果: ジャーナルへの寄稿記事

22 引用 (Scopus)

抄録

The induction of the decidualization of endometrial stromal cells is possible in an in vitro cell culture system. However, thus far, methods differ according to species or cell type, and a more stable or universal system has not yet been developed. The purpose of the present study has been to establish an in vitro decidualization system in primary cultured rat endometrial stromal cells (RES). The RES were treated with medroxyprogesterone acetate and dibutyryl-cyclic adenosine monophosphate (MPA treatment), estradiol and progesterone, or arachidonic acid. After 24 h of treatment, cells responded to all of the stimulations by expressing desmin mRNA. However, decidual/trophoblast prolactin-related protein (dPRP) mRNA was only expressed in the MPA-treated cells. Desmin and dPRP mRNA were not expressed after MPA treatment of the RES derived from immature rat uteri. However, mRNA from both desmin and dPRP were expressed in RES derived from gonadotrophin-injected immature rats. The expression of matrix metalloprotei-nase-2 (MMP-2) and MMP-9 mRNA did not change after the decidual treatment of RES examined by real-time polymerase chain reaction. However, the results of gelatin zymography showed that the active forms of MMP-2 and MMP-9 significantly increased after in vitro decidualization (P<0.05). We conclude that MPA treatment is the most effective method for stimulating decidualization in RES. Use of this system has revealed that sexual maturation and gonadotrophins are important for RES with regard to decidualization. Furthermore, the activity of MMP-2 and MMP-9 might increase during decidualization without a corresponding increase of the expression of these genes.

元の言語英語
ページ(範囲)575-583
ページ数9
ジャーナルCell and tissue research
335
発行部数3
DOI
出版物ステータス出版済み - 3 1 2009

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Stromal Cells
Desmin
Trophoblasts
Messenger RNA
Matrix Metalloproteinases
Prolactin
Gonadotropins
In Vitro Techniques
Sexual Maturation
Medroxyprogesterone Acetate
Proteins
Gelatin
Arachidonic Acid
Cyclic AMP
Uterus
Progesterone
Real-Time Polymerase Chain Reaction
Estradiol
Cell Culture Techniques
Gene Expression

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

これを引用

Matsumoto, K., Yamauchi, N., Watanabe, R., Oozono, S., Kubota, K., Nishimura, K., ... Hattori, M. A. (2009). In vitro decidualization of rat endometrial stromal cells. Cell and tissue research, 335(3), 575-583. https://doi.org/10.1007/s00441-008-0734-1

In vitro decidualization of rat endometrial stromal cells. / Matsumoto, Kenji; Yamauchi, Nobuhiko; Watanabe, Ryo; Oozono, Shinji; Kubota, Kaiyu; Nishimura, Kyohei; Wood, Chris; Soh, Tomoki; Kizaki, Kei Ichirou; Hattori, Masa Aki.

:: Cell and tissue research, 巻 335, 番号 3, 01.03.2009, p. 575-583.

研究成果: ジャーナルへの寄稿記事

Matsumoto, K, Yamauchi, N, Watanabe, R, Oozono, S, Kubota, K, Nishimura, K, Wood, C, Soh, T, Kizaki, KI & Hattori, MA 2009, 'In vitro decidualization of rat endometrial stromal cells', Cell and tissue research, 巻. 335, 番号 3, pp. 575-583. https://doi.org/10.1007/s00441-008-0734-1
Matsumoto K, Yamauchi N, Watanabe R, Oozono S, Kubota K, Nishimura K その他. In vitro decidualization of rat endometrial stromal cells. Cell and tissue research. 2009 3 1;335(3):575-583. https://doi.org/10.1007/s00441-008-0734-1
Matsumoto, Kenji ; Yamauchi, Nobuhiko ; Watanabe, Ryo ; Oozono, Shinji ; Kubota, Kaiyu ; Nishimura, Kyohei ; Wood, Chris ; Soh, Tomoki ; Kizaki, Kei Ichirou ; Hattori, Masa Aki. / In vitro decidualization of rat endometrial stromal cells. :: Cell and tissue research. 2009 ; 巻 335, 番号 3. pp. 575-583.
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abstract = "The induction of the decidualization of endometrial stromal cells is possible in an in vitro cell culture system. However, thus far, methods differ according to species or cell type, and a more stable or universal system has not yet been developed. The purpose of the present study has been to establish an in vitro decidualization system in primary cultured rat endometrial stromal cells (RES). The RES were treated with medroxyprogesterone acetate and dibutyryl-cyclic adenosine monophosphate (MPA treatment), estradiol and progesterone, or arachidonic acid. After 24 h of treatment, cells responded to all of the stimulations by expressing desmin mRNA. However, decidual/trophoblast prolactin-related protein (dPRP) mRNA was only expressed in the MPA-treated cells. Desmin and dPRP mRNA were not expressed after MPA treatment of the RES derived from immature rat uteri. However, mRNA from both desmin and dPRP were expressed in RES derived from gonadotrophin-injected immature rats. The expression of matrix metalloprotei-nase-2 (MMP-2) and MMP-9 mRNA did not change after the decidual treatment of RES examined by real-time polymerase chain reaction. However, the results of gelatin zymography showed that the active forms of MMP-2 and MMP-9 significantly increased after in vitro decidualization (P<0.05). We conclude that MPA treatment is the most effective method for stimulating decidualization in RES. Use of this system has revealed that sexual maturation and gonadotrophins are important for RES with regard to decidualization. Furthermore, the activity of MMP-2 and MMP-9 might increase during decidualization without a corresponding increase of the expression of these genes.",
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AU - Kubota, Kaiyu

AU - Nishimura, Kyohei

AU - Wood, Chris

AU - Soh, Tomoki

AU - Kizaki, Kei Ichirou

AU - Hattori, Masa Aki

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AB - The induction of the decidualization of endometrial stromal cells is possible in an in vitro cell culture system. However, thus far, methods differ according to species or cell type, and a more stable or universal system has not yet been developed. The purpose of the present study has been to establish an in vitro decidualization system in primary cultured rat endometrial stromal cells (RES). The RES were treated with medroxyprogesterone acetate and dibutyryl-cyclic adenosine monophosphate (MPA treatment), estradiol and progesterone, or arachidonic acid. After 24 h of treatment, cells responded to all of the stimulations by expressing desmin mRNA. However, decidual/trophoblast prolactin-related protein (dPRP) mRNA was only expressed in the MPA-treated cells. Desmin and dPRP mRNA were not expressed after MPA treatment of the RES derived from immature rat uteri. However, mRNA from both desmin and dPRP were expressed in RES derived from gonadotrophin-injected immature rats. The expression of matrix metalloprotei-nase-2 (MMP-2) and MMP-9 mRNA did not change after the decidual treatment of RES examined by real-time polymerase chain reaction. However, the results of gelatin zymography showed that the active forms of MMP-2 and MMP-9 significantly increased after in vitro decidualization (P<0.05). We conclude that MPA treatment is the most effective method for stimulating decidualization in RES. Use of this system has revealed that sexual maturation and gonadotrophins are important for RES with regard to decidualization. Furthermore, the activity of MMP-2 and MMP-9 might increase during decidualization without a corresponding increase of the expression of these genes.

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