In Vitro Development of Mouse Embryonic Stem Cells Lacking JNK/Stress-activated Protein Kinase-associated Protein 1 (JSAP1) Scaffold Protein Revealed Its Requirement during Early Embryonic Neurogenesis

Ping Xu, Katsuji Yoshioka, Daisuke Yoshimura, Yohei Tominaga, Tomoko Nishioka, Michihiko Ito, Yusaku Nakabeppu

研究成果: ジャーナルへの寄稿学術誌査読

24 被引用数 (Scopus)

抄録

The Jsap1 gene encodes a scaffold protein for c-Jun N-terminal kinase cascades. We established c-Jun N-terminal kinase (JNK)/stress-activated protein kinase-associated protein 1 (JSAP1)-null mouse embryonic stem cell lines by homologous recombination. The JSAP1-null embryonic stem cells were viable, however, exhibited hyperplasia of the ectoderm during embryoid body formation, and spontaneously differentiated into neurons more efficiently than did wild type. The expression of components of c-Jun N-terminal kinase cascades and a subset of marker mRNAs during early embryogenesis was altered in the JSAP1-null mutants. Retinoic acid dramatically increased the expression of JSAP1 and JNK3, which were co-precipitated with anti-JNK3 in the neuroectoderm of wild type but not JSAP1-null embryoid bodies. In the neurons differentiated from the wild type embryoid bodies, JSAP1 was localized in the soma, neurites, and growth cone-like structure of the neurites, and neurite outgrowth from the JSAP1-null embryoid bodies was apparently less efficient than from wild type. JSAP1 and c-Jun N-terminal kinase 3 were coexpressed in the embryonic ectoderm of E7.5 mouse embryo, whereas Wnt1 and Pax2 were coexpressed with JSAP1 at the midbrain-hindbrain junction in E12.5 mouse embryo, thus suggesting that JSAP1 is required for early embryonic neurogenesis.

本文言語英語
ページ(範囲)48422-48433
ページ数12
ジャーナルJournal of Biological Chemistry
278
48
DOI
出版ステータス出版済み - 11月 28 2003

!!!All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学
  • 細胞生物学

フィンガープリント

「In Vitro Development of Mouse Embryonic Stem Cells Lacking JNK/Stress-activated Protein Kinase-associated Protein 1 (JSAP1) Scaffold Protein Revealed Its Requirement during Early Embryonic Neurogenesis」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル