In Vitro Effects of Methylsulfonyl Polychlorinated Biphenyls and 7,8‐Benzoflavone on Aryl Hydrocarbon Hydroxylase Activity in Human Lymphoblastoid Cells

Chikako Kiyohara, Noriko Mohri, Tomio Hirohata, Koichi Haraguchi, Yoshito Masuda

研究成果: ジャーナルへの寄稿記事

21 引用 (Scopus)

抄録

The effects of 11 isomers of methylsulfonyl polychlorinated biphenyls (MSF‐PCBs) on the aryl hydrocarbon hydroxylase (AHH) activity were examined at a fixed dose of 1.5 μg/ml by using cultured lymphoblastoid cells. One of the isomers 3‐MSF‐3′,4,4′,5‐tetraCB, as well as 4‐MSF‐3,3′,4′,5‐tetraCB and 4‐MSF‐3,3′,4′,5,5′‐pentaCB, effectively reduced the AHH activity when added to the culture medium, especially that previously treated with 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin (TCDD) to induce the AHH activity. However, the inhibitory potencies exerted by the above three MSF‐PCB compounds were smaller than that by 7,8‐benzoflavone (BNF). When added to the culture medium simultaneously with TCDD or 3‐methylcholanthrene (MC), 3‐MSF‐3′,4,4′,5‐tetraCB blocked the enhancement of the AHH activity by TCDD but did not that by MC. This is in contrast to BNF which blocked the increases due to both TCDD and MC. On the other hand, 3‐MSF‐3′,4,4′,5‐tetraCB added directly to the reaction mixture for the TCDD‐induced AHH activity showed little influence, while BNF decreased the same activity. These results imply that the effect of 3‐MSF‐3′,4,4′,5‐tetraCB on the AHH activity is different from that of BNF. 1990 Nordic Pharmacological Society

元の言語英語
ページ(範囲)273-276
ページ数4
ジャーナルPharmacology & Toxicology
66
発行部数4
DOI
出版物ステータス出版済み - 4 1990

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Aryl Hydrocarbon Hydroxylases
Polychlorinated Biphenyls
Human Activities
Isomers
Culture Media
Biphenyl Compounds
In Vitro Techniques
Cultured Cells
Pharmacology
Polychlorinated Dibenzodioxins

All Science Journal Classification (ASJC) codes

  • Toxicology
  • Pharmacology
  • Health, Toxicology and Mutagenesis

これを引用

In Vitro Effects of Methylsulfonyl Polychlorinated Biphenyls and 7,8‐Benzoflavone on Aryl Hydrocarbon Hydroxylase Activity in Human Lymphoblastoid Cells. / Kiyohara, Chikako; Mohri, Noriko; Hirohata, Tomio; Haraguchi, Koichi; Masuda, Yoshito.

:: Pharmacology & Toxicology, 巻 66, 番号 4, 04.1990, p. 273-276.

研究成果: ジャーナルへの寄稿記事

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abstract = "The effects of 11 isomers of methylsulfonyl polychlorinated biphenyls (MSF‐PCBs) on the aryl hydrocarbon hydroxylase (AHH) activity were examined at a fixed dose of 1.5 μg/ml by using cultured lymphoblastoid cells. One of the isomers 3‐MSF‐3′,4,4′,5‐tetraCB, as well as 4‐MSF‐3,3′,4′,5‐tetraCB and 4‐MSF‐3,3′,4′,5,5′‐pentaCB, effectively reduced the AHH activity when added to the culture medium, especially that previously treated with 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin (TCDD) to induce the AHH activity. However, the inhibitory potencies exerted by the above three MSF‐PCB compounds were smaller than that by 7,8‐benzoflavone (BNF). When added to the culture medium simultaneously with TCDD or 3‐methylcholanthrene (MC), 3‐MSF‐3′,4,4′,5‐tetraCB blocked the enhancement of the AHH activity by TCDD but did not that by MC. This is in contrast to BNF which blocked the increases due to both TCDD and MC. On the other hand, 3‐MSF‐3′,4,4′,5‐tetraCB added directly to the reaction mixture for the TCDD‐induced AHH activity showed little influence, while BNF decreased the same activity. These results imply that the effect of 3‐MSF‐3′,4,4′,5‐tetraCB on the AHH activity is different from that of BNF. 1990 Nordic Pharmacological Society",
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AB - The effects of 11 isomers of methylsulfonyl polychlorinated biphenyls (MSF‐PCBs) on the aryl hydrocarbon hydroxylase (AHH) activity were examined at a fixed dose of 1.5 μg/ml by using cultured lymphoblastoid cells. One of the isomers 3‐MSF‐3′,4,4′,5‐tetraCB, as well as 4‐MSF‐3,3′,4′,5‐tetraCB and 4‐MSF‐3,3′,4′,5,5′‐pentaCB, effectively reduced the AHH activity when added to the culture medium, especially that previously treated with 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin (TCDD) to induce the AHH activity. However, the inhibitory potencies exerted by the above three MSF‐PCB compounds were smaller than that by 7,8‐benzoflavone (BNF). When added to the culture medium simultaneously with TCDD or 3‐methylcholanthrene (MC), 3‐MSF‐3′,4,4′,5‐tetraCB blocked the enhancement of the AHH activity by TCDD but did not that by MC. This is in contrast to BNF which blocked the increases due to both TCDD and MC. On the other hand, 3‐MSF‐3′,4,4′,5‐tetraCB added directly to the reaction mixture for the TCDD‐induced AHH activity showed little influence, while BNF decreased the same activity. These results imply that the effect of 3‐MSF‐3′,4,4′,5‐tetraCB on the AHH activity is different from that of BNF. 1990 Nordic Pharmacological Society

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