In vitro gene transfer by the use of laser-induced stress wave: Effect of laser irradiation conditions on transfection efficiency

Mitsuhiro Terakawa, Shunichi Sato, Makoto Ogura, Yoshinori Masaki, Maki Uenoyama, Hitoshi Wakisaka, Hiroshi Ashida, Minoru Obara

研究成果: Contribution to journalConference article査読

抄録

Laser-mediated gene transfection is very attractive as a new method for targeted gene therapy because of its high spatial controllability of laser energy. Previously, we demonstrated both in vivo and in vitro that plasmid DNA can be transfected by applying nanosecond pulsed laser-induced stress waves (LISW). In this study, we investigated the experimental conditions to increase transfection efficiency in vitro. By applying single-pulse LISW, transfection efficiency was increased with increasing laser fluence. Increase in the number of laser pulses increased transfection efficiency for laser fluences up to 1.3 J/cm2, but at higher fluences (>1.7 J/cm2), efficiency showed saturation tendency. Temperature dependence of transfection efficiency was also investigated.

本文言語英語
論文番号36
ページ(範囲)225-229
ページ数5
ジャーナルProgress in Biomedical Optics and Imaging - Proceedings of SPIE
5695
DOI
出版ステータス出版済み - 8 16 2005
外部発表はい
イベントOptical Interactions with Tissue and Cells XVI - San Jose, CA, 米国
継続期間: 1 24 20051 26 2005

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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