In vitro generation of viral-antigen dependent cytotoxic T-cells from ginbuna crucian carp, Carassius auratus langsdorfii

Tomonori Somamoto, Nobuaki Okamoto, Teruyuki Nakanishi, Mitsuru Ototake, Miki Nakao

研究成果: ジャーナルへの寄稿記事

33 引用 (Scopus)

抄録

Little is known about antigen-specific T-cell responses to viruses in teleosts due to a lack of a suitable experimental system using inbred or clonal animals. In the present study we have successfully induced an in vitro generation of virus-specific cytotoxic T-cells (CTLs) from isogeneic ginbuna crucian carp. Responder cells (primarily lymphocytes) from crucian carp haematopoietic necrosis virus (CHNV)-infected fish were capable of proliferating after stimulation in vitro with CHNV-infected syngeneic stimulator cells (primarily lymphocytes and macrophages). The effector cells collected 8 and 12 days after the in vitro stimulation efficiently lysed CHNV-infected syngeneic cells, but not CHNV-infected allogeneic cells or different virus (EVA)-infected syngeneic cells. Furthermore, in situ hybridization analysis showed that some effector cells binding to a CHNV-infected target were TCRβ or CD8α positive. These results provide evidence that the teleost effector cells generated in vitro correspond to virus-specific CTL and they recognize virus-infected target cells in a similar manner of mammalian counterparts.

元の言語英語
ページ(範囲)26-33
ページ数8
ジャーナルVirology
389
発行部数1-2
DOI
出版物ステータス出版済み - 6 2 2009

Fingerprint

Goldfish
Carps
Viral Antigens
Viruses
T-Lymphocytes
Necrosis
Extravehicular Activity
In Vitro Techniques
Lymphocytes
In Situ Hybridization
Fishes
Macrophages
Antigens

All Science Journal Classification (ASJC) codes

  • Virology

これを引用

In vitro generation of viral-antigen dependent cytotoxic T-cells from ginbuna crucian carp, Carassius auratus langsdorfii. / Somamoto, Tomonori; Okamoto, Nobuaki; Nakanishi, Teruyuki; Ototake, Mitsuru; Nakao, Miki.

:: Virology, 巻 389, 番号 1-2, 02.06.2009, p. 26-33.

研究成果: ジャーナルへの寄稿記事

Somamoto, Tomonori ; Okamoto, Nobuaki ; Nakanishi, Teruyuki ; Ototake, Mitsuru ; Nakao, Miki. / In vitro generation of viral-antigen dependent cytotoxic T-cells from ginbuna crucian carp, Carassius auratus langsdorfii. :: Virology. 2009 ; 巻 389, 番号 1-2. pp. 26-33.
@article{c5cd29938b6e40968fa76e7bd3f3b2fb,
title = "In vitro generation of viral-antigen dependent cytotoxic T-cells from ginbuna crucian carp, Carassius auratus langsdorfii",
abstract = "Little is known about antigen-specific T-cell responses to viruses in teleosts due to a lack of a suitable experimental system using inbred or clonal animals. In the present study we have successfully induced an in vitro generation of virus-specific cytotoxic T-cells (CTLs) from isogeneic ginbuna crucian carp. Responder cells (primarily lymphocytes) from crucian carp haematopoietic necrosis virus (CHNV)-infected fish were capable of proliferating after stimulation in vitro with CHNV-infected syngeneic stimulator cells (primarily lymphocytes and macrophages). The effector cells collected 8 and 12 days after the in vitro stimulation efficiently lysed CHNV-infected syngeneic cells, but not CHNV-infected allogeneic cells or different virus (EVA)-infected syngeneic cells. Furthermore, in situ hybridization analysis showed that some effector cells binding to a CHNV-infected target were TCRβ or CD8α positive. These results provide evidence that the teleost effector cells generated in vitro correspond to virus-specific CTL and they recognize virus-infected target cells in a similar manner of mammalian counterparts.",
author = "Tomonori Somamoto and Nobuaki Okamoto and Teruyuki Nakanishi and Mitsuru Ototake and Miki Nakao",
year = "2009",
month = "6",
day = "2",
doi = "10.1016/j.virol.2009.04.008",
language = "English",
volume = "389",
pages = "26--33",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "1-2",

}

TY - JOUR

T1 - In vitro generation of viral-antigen dependent cytotoxic T-cells from ginbuna crucian carp, Carassius auratus langsdorfii

AU - Somamoto, Tomonori

AU - Okamoto, Nobuaki

AU - Nakanishi, Teruyuki

AU - Ototake, Mitsuru

AU - Nakao, Miki

PY - 2009/6/2

Y1 - 2009/6/2

N2 - Little is known about antigen-specific T-cell responses to viruses in teleosts due to a lack of a suitable experimental system using inbred or clonal animals. In the present study we have successfully induced an in vitro generation of virus-specific cytotoxic T-cells (CTLs) from isogeneic ginbuna crucian carp. Responder cells (primarily lymphocytes) from crucian carp haematopoietic necrosis virus (CHNV)-infected fish were capable of proliferating after stimulation in vitro with CHNV-infected syngeneic stimulator cells (primarily lymphocytes and macrophages). The effector cells collected 8 and 12 days after the in vitro stimulation efficiently lysed CHNV-infected syngeneic cells, but not CHNV-infected allogeneic cells or different virus (EVA)-infected syngeneic cells. Furthermore, in situ hybridization analysis showed that some effector cells binding to a CHNV-infected target were TCRβ or CD8α positive. These results provide evidence that the teleost effector cells generated in vitro correspond to virus-specific CTL and they recognize virus-infected target cells in a similar manner of mammalian counterparts.

AB - Little is known about antigen-specific T-cell responses to viruses in teleosts due to a lack of a suitable experimental system using inbred or clonal animals. In the present study we have successfully induced an in vitro generation of virus-specific cytotoxic T-cells (CTLs) from isogeneic ginbuna crucian carp. Responder cells (primarily lymphocytes) from crucian carp haematopoietic necrosis virus (CHNV)-infected fish were capable of proliferating after stimulation in vitro with CHNV-infected syngeneic stimulator cells (primarily lymphocytes and macrophages). The effector cells collected 8 and 12 days after the in vitro stimulation efficiently lysed CHNV-infected syngeneic cells, but not CHNV-infected allogeneic cells or different virus (EVA)-infected syngeneic cells. Furthermore, in situ hybridization analysis showed that some effector cells binding to a CHNV-infected target were TCRβ or CD8α positive. These results provide evidence that the teleost effector cells generated in vitro correspond to virus-specific CTL and they recognize virus-infected target cells in a similar manner of mammalian counterparts.

UR - http://www.scopus.com/inward/record.url?scp=67349250453&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67349250453&partnerID=8YFLogxK

U2 - 10.1016/j.virol.2009.04.008

DO - 10.1016/j.virol.2009.04.008

M3 - Article

C2 - 19428040

AN - SCOPUS:67349250453

VL - 389

SP - 26

EP - 33

JO - Virology

JF - Virology

SN - 0042-6822

IS - 1-2

ER -