In vitro immunization can elicit the expansion of diverse repertoire of B cells from peripheral blood mononuclear cells

Shin Ei Matsumoto, Makiko Yamashita, Yoshinori Katakura, Eri Noguchi, Yoshihiro Aiba, Akira Ichikawa, Kiichiro Teruya, Sanetaka Shirahata

研究成果: Contribution to journalArticle査読

8 被引用数 (Scopus)

抄録

We previously developed an in vitro immunization (IVI) protocol of human peripheral blood mononuclear cells (PBMC) for generating antigen-specific human antibodies. In order to clarify whether IVI protocolinduces antigen-specific B cell responses in PBMC, we analyzed family gene usage and sequence of the variable region gene of immunoglobulin heavy chain (VH gene) of the antibody produced from the in vitro immunized PBMC. Sequence homology analyses of VH gene demonstrated that a larger repertoire of B cells can be sensitized with mite-extract than with cholera toxin B subunit and rice allergen. Further, antigen-specific B cells were efficiently expanded by using CpG oligodeoxynucleotide as adjuvant. These results suggest that appropriate combination of sensitizing antigen and adjuvant is primarily important for expansion of antigen-specific B cells in IVI protocol.

本文言語英語
ページ(範囲)227-233
ページ数7
ジャーナルCytotechnology
52
3
DOI
出版ステータス出版済み - 11 2006

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • バイオエンジニアリング
  • 生体医工学
  • 臨床生化学
  • 細胞生物学

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