In vitro production of functionally mature hepatocytes from prospectively isolated hepatic stem cells

Atsushi Suzuki, Hiromitsu Nakauchi, Hideki Taniguchi

研究成果: Contribution to journalArticle査読

26 被引用数 (Scopus)

抄録

Hepatocyte transplantation and artificial organ hepatic support require a number of functionally mature hepatocytes. However, their growth activity and functional behaviors are much smaller in culture after isolation from the liver. We examined whether continuously differentiating hepatocytes from multipotent hepatic stem cells that were isolated by using flow cytometry and propagated clonally in culture could be a source of clinical application. They actually gave rise to cells that were functionally equal to mature hepatocytes found in the adult liver, which secreted albumin into culture medium and metabolized harmful ammonium into urea. These data suggest that stem cell-derived hepatocytes are a useful cell source for developing therapeutic strategies, such as cell transplantation, gene therapy, and artificial liver organ to treat various liver disorders.

本文言語英語
ページ(範囲)469-473
ページ数5
ジャーナルCell Transplantation
12
5
DOI
出版ステータス出版済み - 2003
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生体医工学
  • 細胞生物学
  • 移植

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