TY - JOUR
T1 - In vitro reconstitution of germ cell development
AU - Hayashi, Katsuhiko
N1 - Funding Information:
Department of Stem Cell Biology and Medicine, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan ∗Correspondence: Department of Stem Cell Biology and Medicine, Graduate School of Medical Sciences, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan. E-mail: hayashik@hgs.med.kyushu-u.ac.jp †Grant support: This study was supported in part by a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (KAKENHI #18H05544 and #18H05545); by the Uehara Memorial Foundation; and by the Takeda Science Foundation. Edited by Dr. Kyle Orwig, PhD, University of Pittsburgh
Publisher Copyright:
© 2019 The Author(s) 2019. Published by Oxford University Press on behalf of Society for the Study of Reproduction.
PY - 2019/9/1
Y1 - 2019/9/1
N2 - Germ cell development is a series of highly specialized processes through which diploid pluripotent cells differentiate into haploid gametes. The processes include biologically important events such as epigenetic reprogramming, sex determination, and meiosis. The mechanisms underlying these events are key issues in reproductive and developmental biology, yet they still remain elusive. As a tool to elucidate these mechanisms, in vitro gametogenesis, which reproduces germ cell development in culture, has long been sought for decades. Recently, methods of in vitro gametogenesis have undergone rapid development in association with stem cell biology, opening many possibilities in this field. This new technology is considered an alternative source of gametes for the reproduction of animals and perhaps humans. This review summarizes current advances and problems in in vitro gametogenesis.
AB - Germ cell development is a series of highly specialized processes through which diploid pluripotent cells differentiate into haploid gametes. The processes include biologically important events such as epigenetic reprogramming, sex determination, and meiosis. The mechanisms underlying these events are key issues in reproductive and developmental biology, yet they still remain elusive. As a tool to elucidate these mechanisms, in vitro gametogenesis, which reproduces germ cell development in culture, has long been sought for decades. Recently, methods of in vitro gametogenesis have undergone rapid development in association with stem cell biology, opening many possibilities in this field. This new technology is considered an alternative source of gametes for the reproduction of animals and perhaps humans. This review summarizes current advances and problems in in vitro gametogenesis.
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U2 - 10.1093/biolre/ioz111
DO - 10.1093/biolre/ioz111
M3 - Review article
C2 - 31295346
AN - SCOPUS:85073184506
VL - 101
SP - 567
EP - 578
JO - Biology of Reproduction
JF - Biology of Reproduction
SN - 0006-3363
IS - 3
ER -