In vivo angiogenesis imaging of solid tumors by αvβ 3-targeted, dual-modality micellar nanoprobes

Chase W. Kessinger, Chalermchai Khemtong, Osamu Togao, Masaya Takahashi, Baran D. Sumer, Jinming Gao

研究成果: ジャーナルへの寄稿学術誌査読

25 被引用数 (Scopus)

抄録

The objective of this study was to develop and evaluate an αvβ3-specific nanoprobe consisting of fluorescent superparamagnetic polymeric micelles (FSPPM) for in vivo imaging of tumor angiogenesis. Spherical micelles were produced using poly(ethylene glycol)-b-poly(D,L-lactide) co-polymers conjugated with tetramethylrhodamine, a fluorescent dye, and loaded with superparamagnetic iron oxide nanoparticles. The resulting micelle diameter was 50-70 nm by dynamic light scattering and transmission electron microscopy measurements. Micelles were encoded with an αvβ3-specific peptide, cyclic RGDfK, and optimized for maximum fluorescence and targeting in αvβ 3-overexpressing cells in vitro. In mice, cRGD-FSPPM-treated animals showed αvβ3-specific FSPPM accumulation in human lung cancer subcutaneous tumor xenografts. Together with the histological validation, the three-dimensional gradient echo magnetic resonance imaging (MRI) data provide high spatial resolution mapping and quantification of angiogenic vasculature in an animal tumor model using targeted, ultrasensitive MRI nanoprobes.

本文言語英語
ページ(範囲)957-965
ページ数9
ジャーナルExperimental Biology and Medicine
235
8
DOI
出版ステータス出版済み - 8月 2010
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 生化学、遺伝学、分子生物学(全般)

フィンガープリント

「In vivo angiogenesis imaging of solid tumors by αvβ 3-targeted, dual-modality micellar nanoprobes」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル