In vivo fluorescence navigation of gastric and upper gastrointestinal tumors by 5-aminolevulinic acid mediated photodynamic diagnosis with a laser-equipped video image endoscope

Hajime Isomoto, Atsushi Nanashima, Takemasa Senoo, Kumi Ogiwara, Mikiko Hashisako, Ken Ohnita, Naoyuki Yamaguchi, Masaki Kunizaki, Shigekazu Hidaka, Hiroko Fukuda, Hiroyuki Ishii, Kayoko Matsushima, Hitomi Minami, Yuko Akazawa, Fuminao Takeshima, Junya Fukuoka, Takeshi Nagayasu, Kazuhiko Nakao

研究成果: Contribution to journalArticle査読

12 被引用数 (Scopus)

抄録

Photodynamic diagnosis (PDD) is an imaging technology that is based on the fundamental biological features of cancer cells. Five-aminolevulinic acid (ALA), a precursor of photosensitizing protoporphyrin IX (PpIX) has been applied. In fact, ALA-mediated PDD provides good visualization for certain tumors. However, there have been few studies on clinical application of PDD for gastrointestinal (GI) cancers. This study was aimed to investigate the feasibility of ALA-mediated PDD for navigation of upper GI tumors. Materials and methods: Using a newly developed endoscope equipped with a blue laser light excitation, ALA-mediated PDD was conducted in 27 lesions from 23 patients with upper GI tumors including 2 Barrett's intramucosal cancers. ALA solution was given orally 3. h before PDD. All the adenocarcinomas came under clinical stage I, and the tumors were resected endoscopic submucosal dissection and/or laparoscopic surgery. Red fluorescence signal and intensity was assessed as for clinicopathological features of the cases. Results: The laser-based endoscopy could detect upper GI tumors as red fluorescent navigation (PDD-positive) in 23 of the 27 lesions. All but one intestinal typed tumors in histopathology were significantly PDD-positive, whereas each signet ring cell carcinoma was PDD-negative. There was a significant difference in tumor size between the PDD-positive and -negative tumors. The elevated lesions emitted significantly more intense fluorescence. Conclusions: Fluorescence navigation by ALA-mediated PDD provided sufficient detection of upper GI tumors in particular for the intestinal typed tumors. Thus, ALA-PDD using the blue laser-equipped endoscope offers a promising diagnostic tool.

本文言語英語
ページ(範囲)201-208
ページ数8
ジャーナルPhotodiagnosis and Photodynamic Therapy
12
2
DOI
出版ステータス出版済み - 6 1 2015
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 腫瘍学
  • 皮膚病学
  • 薬理学(医学)

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