In vivo magnetic resonance imaging of atherosclerotic lesions with a newly developed evans blue-DTPA-gadolinium contrast medium in apolipoprotein-E- deficient mice

Satoshi Yasuda, Kenjiro Ikuta, Toyokazu Uwatoku, Keiji Oi, Kotaro Abe, Fuminori Hyodo, Kengo Yoshimitsu, Kohtaro Sugimura, Hideo Utsumi, Yoshiki Katayama, Hiroaki Shimokawa

研究成果: ジャーナルへの寄稿記事

7 引用 (Scopus)

抄録

Background: Magnetic resonance imaging (MRI) contrast agents that specifically detect atherosclerotic plaque may be useful for the noninvasive detection of the plaque. We have recently developed a new contrast agent, Evans blue-DTPA-gadolinium (EB-DTPA-Gd), which selectively accumulates vascular lesions with endothelial removal. In this study, we examined whether EB-DTPA-Gd is also useful for in vivo imaging of atherosclerotic plaques. Methods: We used male apolipoprotein-E-deficient (ApoE-/-) mice of different ages (3, 6 and 12 months old) and age-matched male wild-type mice. After a single intravenous administration of EB-DTPA-Gd (160 μM/kg body weight), MRI T1 signal was obtained in vivo. Results: Increased signal intensity in the aortic wall was noted within 10-20 min after intravenous injection of EB-DTPA-Gd and was maintained for 30 min. The MRI enhancement in the aorta of ApoE-/- mice was increased in accordance with age, whereas no such enhancement was noted in wild-type mice. Histological examination demonstrated that there was a topological correlation between the site of MRI enhancement and that of atherosclerotic plaque. Conclusions: These results indicate that EB-DTPA-Gd is a useful MRI contrast medium for the in vivo detection of atherosclerotic plaques.

元の言語英語
ページ(範囲)123-128
ページ数6
ジャーナルJournal of Vascular Research
45
発行部数2
DOI
出版物ステータス出版済み - 2 1 2008

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Apolipoproteins E
Contrast Media
Atherosclerotic Plaques
Magnetic Resonance Imaging
Intravenous Injections
Intravenous Administration
Blood Vessels
Aorta
Body Weight
Evans blue-DTPA-gadolinium

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cardiology and Cardiovascular Medicine

これを引用

In vivo magnetic resonance imaging of atherosclerotic lesions with a newly developed evans blue-DTPA-gadolinium contrast medium in apolipoprotein-E- deficient mice. / Yasuda, Satoshi; Ikuta, Kenjiro; Uwatoku, Toyokazu; Oi, Keiji; Abe, Kotaro; Hyodo, Fuminori; Yoshimitsu, Kengo; Sugimura, Kohtaro; Utsumi, Hideo; Katayama, Yoshiki; Shimokawa, Hiroaki.

:: Journal of Vascular Research, 巻 45, 番号 2, 01.02.2008, p. 123-128.

研究成果: ジャーナルへの寄稿記事

Yasuda, Satoshi ; Ikuta, Kenjiro ; Uwatoku, Toyokazu ; Oi, Keiji ; Abe, Kotaro ; Hyodo, Fuminori ; Yoshimitsu, Kengo ; Sugimura, Kohtaro ; Utsumi, Hideo ; Katayama, Yoshiki ; Shimokawa, Hiroaki. / In vivo magnetic resonance imaging of atherosclerotic lesions with a newly developed evans blue-DTPA-gadolinium contrast medium in apolipoprotein-E- deficient mice. :: Journal of Vascular Research. 2008 ; 巻 45, 番号 2. pp. 123-128.
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abstract = "Background: Magnetic resonance imaging (MRI) contrast agents that specifically detect atherosclerotic plaque may be useful for the noninvasive detection of the plaque. We have recently developed a new contrast agent, Evans blue-DTPA-gadolinium (EB-DTPA-Gd), which selectively accumulates vascular lesions with endothelial removal. In this study, we examined whether EB-DTPA-Gd is also useful for in vivo imaging of atherosclerotic plaques. Methods: We used male apolipoprotein-E-deficient (ApoE-/-) mice of different ages (3, 6 and 12 months old) and age-matched male wild-type mice. After a single intravenous administration of EB-DTPA-Gd (160 μM/kg body weight), MRI T1 signal was obtained in vivo. Results: Increased signal intensity in the aortic wall was noted within 10-20 min after intravenous injection of EB-DTPA-Gd and was maintained for 30 min. The MRI enhancement in the aorta of ApoE-/- mice was increased in accordance with age, whereas no such enhancement was noted in wild-type mice. Histological examination demonstrated that there was a topological correlation between the site of MRI enhancement and that of atherosclerotic plaque. Conclusions: These results indicate that EB-DTPA-Gd is a useful MRI contrast medium for the in vivo detection of atherosclerotic plaques.",
author = "Satoshi Yasuda and Kenjiro Ikuta and Toyokazu Uwatoku and Keiji Oi and Kotaro Abe and Fuminori Hyodo and Kengo Yoshimitsu and Kohtaro Sugimura and Hideo Utsumi and Yoshiki Katayama and Hiroaki Shimokawa",
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AU - Uwatoku, Toyokazu

AU - Oi, Keiji

AU - Abe, Kotaro

AU - Hyodo, Fuminori

AU - Yoshimitsu, Kengo

AU - Sugimura, Kohtaro

AU - Utsumi, Hideo

AU - Katayama, Yoshiki

AU - Shimokawa, Hiroaki

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N2 - Background: Magnetic resonance imaging (MRI) contrast agents that specifically detect atherosclerotic plaque may be useful for the noninvasive detection of the plaque. We have recently developed a new contrast agent, Evans blue-DTPA-gadolinium (EB-DTPA-Gd), which selectively accumulates vascular lesions with endothelial removal. In this study, we examined whether EB-DTPA-Gd is also useful for in vivo imaging of atherosclerotic plaques. Methods: We used male apolipoprotein-E-deficient (ApoE-/-) mice of different ages (3, 6 and 12 months old) and age-matched male wild-type mice. After a single intravenous administration of EB-DTPA-Gd (160 μM/kg body weight), MRI T1 signal was obtained in vivo. Results: Increased signal intensity in the aortic wall was noted within 10-20 min after intravenous injection of EB-DTPA-Gd and was maintained for 30 min. The MRI enhancement in the aorta of ApoE-/- mice was increased in accordance with age, whereas no such enhancement was noted in wild-type mice. Histological examination demonstrated that there was a topological correlation between the site of MRI enhancement and that of atherosclerotic plaque. Conclusions: These results indicate that EB-DTPA-Gd is a useful MRI contrast medium for the in vivo detection of atherosclerotic plaques.

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