TY - GEN
T1 - In vivo spectral-spatial imaging for oxygen mapping using single-point, time-domain electron paramagnetic resonance
AU - Subramanian, Sankaran
AU - Johnson, Calvin A.
AU - Devasahayam, Nallathamby
AU - Matsumoto, Ken Ichiro
AU - Hyodo, Fuminori
AU - Cook, John
AU - Krishna, Murali C.
PY - 2006/11/17
Y1 - 2006/11/17
N2 - Electron Paramagnetic Resonance (EPR) is a spectroscopic technique that detects and characterizes molecules with unpaired electrons (i.e., free radicals). Although it is closely related to nuclear magnetic resonance (NMR) spectroscopy, EPR is still under development as an imaging modality. Unlike other imaging modalities, EPR is able to take direct measurements of tissue oxygen concentration in a manner that is not dependent on complex biological processes such as ligand binding specificity or tracer metabolism. We describe the use of single-point imaging (SPI) in radiofrequency, Fourier-transform EPR. We present the techniques for performing oxygen imaging using EPR with phantoms and in vivo. We present results from a study in which the two mouse legs (one normal and the other with tumor) were imaged over time as the mouse breathed alternately air, carbogen (95% O 2, 5% CO 2), and then air again. The reconstructed images demonstrate that the SPI EPR imaging technique readily distinguishes between the normal and tumor legs and can track the changes in tissue oxygen concentration in response to percentage of oxygen in breathing gas.
AB - Electron Paramagnetic Resonance (EPR) is a spectroscopic technique that detects and characterizes molecules with unpaired electrons (i.e., free radicals). Although it is closely related to nuclear magnetic resonance (NMR) spectroscopy, EPR is still under development as an imaging modality. Unlike other imaging modalities, EPR is able to take direct measurements of tissue oxygen concentration in a manner that is not dependent on complex biological processes such as ligand binding specificity or tracer metabolism. We describe the use of single-point imaging (SPI) in radiofrequency, Fourier-transform EPR. We present the techniques for performing oxygen imaging using EPR with phantoms and in vivo. We present results from a study in which the two mouse legs (one normal and the other with tumor) were imaged over time as the mouse breathed alternately air, carbogen (95% O 2, 5% CO 2), and then air again. The reconstructed images demonstrate that the SPI EPR imaging technique readily distinguishes between the normal and tumor legs and can track the changes in tissue oxygen concentration in response to percentage of oxygen in breathing gas.
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M3 - Conference contribution
AN - SCOPUS:33750934161
SN - 0780395778
SN - 9780780395770
T3 - 2006 3rd IEEE International Symposium on Biomedical Imaging: From Nano to Macro - Proceedings
SP - 1096
EP - 1099
BT - 2006 3rd IEEE International Symposium on Biomedical Imaging
T2 - 2006 3rd IEEE International Symposium on Biomedical Imaging: From Nano to Macro
Y2 - 6 April 2006 through 9 April 2006
ER -