Inactivation of enzymes in an aqueous solution by micro-bubbles of supercritical carbon dioxide

Hiroya Ishikawa, Mitsuya Shimoda, Tamotsu Kawano, Yutaka Osajima

研究成果: ジャーナルへの寄稿記事

抜粋

Enzyme solutions of glucoamylase, acid protease, alkaline protease and lipase were treated with micro-bubbles of supercritical carbon dioxide (SC CO2) fed from a cylindrical filter nozzle. The micro-bubbles of SC CO2 could increase the CO2 concentration in the sample solution from 0.4 to 0.92 mol/l at 25 MPa and 35°C, and hence could improve the efficiency of inactivation by about 3 times compared to treating without the filter nozzle. Alkaline protease and lipase in the solution could be completely inactivated by the treatment at 35°C and 15 MPa for 30 min. With the inactivation of glucoamylase and acid protease, their residual activity-CO2 density profiles consisted of two straight lines with intersections at density values of 0.82 and 0.60 g/cm3, respectively. These enzymes showed an abrupt decrease in activation in regions above the CO2 density of their intersections.

元の言語英語
ページ(範囲)628-631
ページ数4
ジャーナルBioscience, Biotechnology and Biochemistry
59
発行部数4
DOI
出版物ステータス出版済み - 1 1 1995

    フィンガープリント

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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