Inactivation of Enzymes in an Aqueous Solution by Micro-Bubbles of Supercritical Carbon Dioxide

Hiroya Ishikawa, Mitsuya Shimoda, Yutaka Osajima, Tamotsu Kawano

研究成果: Contribution to journalArticle査読

58 被引用数 (Scopus)

抄録

Enzyme solutions of glucoamylase, acid protease, alkaline protease and lipase were treated with micro-bubbles of supercritical carbon dioxide (SC CO2) fed from a cylindrical filter nozzle. The micro-bubbles of SC CO2 could increase the CO2 concentration in the sample solution from 0.4 to 0.92 mol/1 at 25 MPa and 35°C, and hence could improve the efficiency of inactivation by about 3 times compared to treating without the filter nozzle. Alkaline protease and lipase in the solution could be completely inactivated by the treatment at 35°C and 15 MPa for 30 min. With the inactivation of glucoamylase and acid protease, their residual activity-CO2 density profiles consisted of two straight lines with intersections at density values of 0.82 and 0.60 g/cm3. respectively. These enzymes above the CO2 density of their intersections.

本文言語英語
ページ(範囲)628-631
ページ数4
ジャーナルBioscience, biotechnology, and biochemistry
59
4
DOI
出版ステータス出版済み - 1995

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 分析化学
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 分子生物学
  • 有機化学

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