Inclusive estimation of complex antigen presentation functions of monocyte-derived dendritic cells differentiated under normoxia and hypoxia conditions

Toshitatsu Ogino, Hideya Ohnishi, Hiroyuki Suzuki, Takashi Morisaki, Masao Tanaka, Mitsuo Katano

研究成果: ジャーナルへの寄稿記事

14 引用 (Scopus)

抄録

Dendritic cells (DCs) generated from monocytes under 20% O 2 are now used as therapeutic tools for cancer patients. However, the O 2 concentration is between 3 and 0.5% in most tissues. We evaluated these complicated functions of DCs under oxygen tensions mimicking in vivo situations. Immature DCs (imDCs) were generated from monocytes using IL-4 and GM-CSF under normoxia (20% O 2; N-imDCs) or hypoxia (1% O 2; H-imDCs). Mature DCs (mDCs) were induced with LPS. DCs were further exposed to normoxia (N/N-DCs) or hypoxia (N/H-DCs and H/H-DCs) conditions. Using a 2-D culture system, H-DCs were smaller in size than N-DCs, and H/HDCs exhibited higher allo-T cell stimulation ability than N/N-DCs and N/H-DCs. On the other hand, motility and phagocytic ability of H/H-DCs were significantly lower than those of N/H-DCs and N/N-DCs. In a 3-D culture system, however, maturation of H/H-imDCs and N/H-im- DCs was suppressed compared with N/N-imDCs as a result of their decreased motility and phagocytosis. Interestingly, silencing of HIF-1α by RNA interference decreased CD83 expression without affecting any antigen presentation abilities except for the ability to stimulate the allo-T cell population. Our data could help our understanding of DCs, especially therapeutic DCs, in vivo.

元の言語英語
ページ(範囲)409-424
ページ数16
ジャーナルCancer Immunology, Immunotherapy
61
発行部数3
DOI
出版物ステータス出版済み - 3 1 2012

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Antigen Presentation
Dendritic Cells
Monocytes
Hypoxia
T-Lymphocytes
Cell Hypoxia
Granulocyte-Macrophage Colony-Stimulating Factor
RNA Interference

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Oncology
  • Cancer Research

これを引用

Inclusive estimation of complex antigen presentation functions of monocyte-derived dendritic cells differentiated under normoxia and hypoxia conditions. / Ogino, Toshitatsu; Ohnishi, Hideya; Suzuki, Hiroyuki; Morisaki, Takashi; Tanaka, Masao; Katano, Mitsuo.

:: Cancer Immunology, Immunotherapy, 巻 61, 番号 3, 01.03.2012, p. 409-424.

研究成果: ジャーナルへの寄稿記事

Ogino, Toshitatsu ; Ohnishi, Hideya ; Suzuki, Hiroyuki ; Morisaki, Takashi ; Tanaka, Masao ; Katano, Mitsuo. / Inclusive estimation of complex antigen presentation functions of monocyte-derived dendritic cells differentiated under normoxia and hypoxia conditions. :: Cancer Immunology, Immunotherapy. 2012 ; 巻 61, 番号 3. pp. 409-424.
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abstract = "Dendritic cells (DCs) generated from monocytes under 20{\%} O 2 are now used as therapeutic tools for cancer patients. However, the O 2 concentration is between 3 and 0.5{\%} in most tissues. We evaluated these complicated functions of DCs under oxygen tensions mimicking in vivo situations. Immature DCs (imDCs) were generated from monocytes using IL-4 and GM-CSF under normoxia (20{\%} O 2; N-imDCs) or hypoxia (1{\%} O 2; H-imDCs). Mature DCs (mDCs) were induced with LPS. DCs were further exposed to normoxia (N/N-DCs) or hypoxia (N/H-DCs and H/H-DCs) conditions. Using a 2-D culture system, H-DCs were smaller in size than N-DCs, and H/HDCs exhibited higher allo-T cell stimulation ability than N/N-DCs and N/H-DCs. On the other hand, motility and phagocytic ability of H/H-DCs were significantly lower than those of N/H-DCs and N/N-DCs. In a 3-D culture system, however, maturation of H/H-imDCs and N/H-im- DCs was suppressed compared with N/N-imDCs as a result of their decreased motility and phagocytosis. Interestingly, silencing of HIF-1α by RNA interference decreased CD83 expression without affecting any antigen presentation abilities except for the ability to stimulate the allo-T cell population. Our data could help our understanding of DCs, especially therapeutic DCs, in vivo.",
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