Increase in Ca2+ permeability of intracellular Ca2+ store membrane of saponin-treated Guinea pig peritoneal macrophages by inositol 1,4,5-trisphosphate

Masato Hirata, Masataka Kukita, Toshiyuki Sasaguri, Eiichi Suematsu, Toshihiko Hashimoto, Toshitaka Koga

研究成果: Contribution to journalArticle査読

17 被引用数 (Scopus)

抄録

Inositol 1,4,5-trisphosphate (InsP3) releases Ca2+ from the non-mitochondrial Ca2+ store site of various types of cells. To study the mechanisms of the Ca2+ release from the store site, the effect of InsP3 on the passive Ca2+ release and influx, and the active Ca2+ uptake in the presence of oxalate, was examined using saponintreated Guinea pig peritoneal macrophages. InsP3 stimulated the passive Ca2+ release and influx. Although InsP3 slightly inhibited the active Ca2+ uptake in the presence of oxalate, it seems unlikely that the Ca2+ release by this agent is caused by the inhibition of the Ca2+ uptake, because the addition of apyrase or hexokinase (which removes ATP within 30 s, so that no more Ca2+ can be accumulated) or vanadate (which inhibits the Ca2+ uptake) resulted in very slow release of Ca2+. These results suggest that the Ca2+ permeability of the Ca2+ store membrane is increased by InsP3. InsP3 did not cause an increase in the Ca2+ permeability of phospholipid vesicles (liposomes), indicating that this agent may bring about Ca2+ release by a specific effect on the physiologically relevant Ca2+ channels or carriers in the non-mitochondrial Ca2+ store site. The passive Ca2+ release by InsP3 was enhanced by ATP and an unhydrolyzable ATP analogue, 5'-adenylyimidodiphosphate, but not by ADP or AMP. The passive Ca2+ release by InsP3 was observed even at 0°C.

本文言語英語
ページ(範囲)1575-1582
ページ数8
ジャーナルJournal of biochemistry
97
6
DOI
出版ステータス出版済み - 6 1985

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学

フィンガープリント

「Increase in Ca<sup>2+</sup> permeability of intracellular Ca<sup>2+</sup> store membrane of saponin-treated Guinea pig peritoneal macrophages by inositol 1,4,5-trisphosphate」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

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