Increase in hydrophobicity of Bacillus subtilis spores by heat, hydrostatic pressure, and pressurized carbon dioxide treatments

Seiji Noma, Kazuki Kiyohara, Rina Hirokado, Nami Yamashita, Yuya Migita, Motoharu Tanaka, Soichi Furukawa, Hirokazu Ogihara, Yasushi Morinaga, Noriyuki Igura, Mitsuya Shimoda

研究成果: ジャーナルへの寄稿記事

2 引用 (Scopus)

抄録

The effects of heat treatment (HT), hydrostatic pressure treatment (HPT), and pressurized carbon dioxide treatment (CT) on surface hydrophobicity of B. subtilis 168 spores were investigated. The spore surface hydrophobicity was measured by determining the ratio of hydrophobic spores (RHS) that were partitioned into the n-hexadecane phase from the aqueous spore suspension. The RHS after HT generally increased in a temperature-dependent manner and reached approximately 10% at temperatures above 60°C. The effects of pressurization by HPT and accompanying temperature on increased RHS were complex. The highest RHS after HPT was approximately 17%. Following CT, RHS reached approximately 80% at 5 MPa at 80°C for 30 min. An increased treatment temperature enhanced RHS by CT. The increase in RHS by CT led to the formation of spore clumps and adhesion of spores to hydrophobic surfaces. Acidification of spore suspension to pH 3.2, expected pH during CT, by HCl also increased the adhesion of spores at the similar degree with CT. The spore surface zeta potential distribution was not changed by CT. Furthermore, spores with increased RHS after CT had germination-like phenomena including loss of their refractility and enhanced staining by 4′,6-diamidino-2-phenylindole. Physiological germination that was induced by the addition of L-alanine also increased the RHS. From these results, it is clear that CT under heating considerably increases RHS. CT under heating considerably increases RHS. This increase in RHS may be due to acidification or germination-like phenomena during CT.

元の言語英語
ページ(範囲)327-332
ページ数6
ジャーナルJournal of Bioscience and Bioengineering
125
発行部数3
DOI
出版物ステータス出版済み - 3 1 2018

Fingerprint

Hydrostatic Pressure
Bacilli
Hydrostatic pressure
Hydrophobicity
Bacillus subtilis
Spores
Hydrophobic and Hydrophilic Interactions
Carbon Dioxide
Carbon dioxide
Hot Temperature
Acidification
Suspensions
Germination
Adhesion
Heat treatment
Heating
Temperature
Pressurization
Zeta potential
Alanine

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

これを引用

Increase in hydrophobicity of Bacillus subtilis spores by heat, hydrostatic pressure, and pressurized carbon dioxide treatments. / Noma, Seiji; Kiyohara, Kazuki; Hirokado, Rina; Yamashita, Nami; Migita, Yuya; Tanaka, Motoharu; Furukawa, Soichi; Ogihara, Hirokazu; Morinaga, Yasushi; Igura, Noriyuki; Shimoda, Mitsuya.

:: Journal of Bioscience and Bioengineering, 巻 125, 番号 3, 01.03.2018, p. 327-332.

研究成果: ジャーナルへの寄稿記事

Noma, S, Kiyohara, K, Hirokado, R, Yamashita, N, Migita, Y, Tanaka, M, Furukawa, S, Ogihara, H, Morinaga, Y, Igura, N & Shimoda, M 2018, 'Increase in hydrophobicity of Bacillus subtilis spores by heat, hydrostatic pressure, and pressurized carbon dioxide treatments', Journal of Bioscience and Bioengineering, 巻. 125, 番号 3, pp. 327-332. https://doi.org/10.1016/j.jbiosc.2017.09.012
Noma, Seiji ; Kiyohara, Kazuki ; Hirokado, Rina ; Yamashita, Nami ; Migita, Yuya ; Tanaka, Motoharu ; Furukawa, Soichi ; Ogihara, Hirokazu ; Morinaga, Yasushi ; Igura, Noriyuki ; Shimoda, Mitsuya. / Increase in hydrophobicity of Bacillus subtilis spores by heat, hydrostatic pressure, and pressurized carbon dioxide treatments. :: Journal of Bioscience and Bioengineering. 2018 ; 巻 125, 番号 3. pp. 327-332.
@article{42581f1c90da4cef9f86f4e955a49a50,
title = "Increase in hydrophobicity of Bacillus subtilis spores by heat, hydrostatic pressure, and pressurized carbon dioxide treatments",
abstract = "The effects of heat treatment (HT), hydrostatic pressure treatment (HPT), and pressurized carbon dioxide treatment (CT) on surface hydrophobicity of B. subtilis 168 spores were investigated. The spore surface hydrophobicity was measured by determining the ratio of hydrophobic spores (RHS) that were partitioned into the n-hexadecane phase from the aqueous spore suspension. The RHS after HT generally increased in a temperature-dependent manner and reached approximately 10{\%} at temperatures above 60°C. The effects of pressurization by HPT and accompanying temperature on increased RHS were complex. The highest RHS after HPT was approximately 17{\%}. Following CT, RHS reached approximately 80{\%} at 5 MPa at 80°C for 30 min. An increased treatment temperature enhanced RHS by CT. The increase in RHS by CT led to the formation of spore clumps and adhesion of spores to hydrophobic surfaces. Acidification of spore suspension to pH 3.2, expected pH during CT, by HCl also increased the adhesion of spores at the similar degree with CT. The spore surface zeta potential distribution was not changed by CT. Furthermore, spores with increased RHS after CT had germination-like phenomena including loss of their refractility and enhanced staining by 4′,6-diamidino-2-phenylindole. Physiological germination that was induced by the addition of L-alanine also increased the RHS. From these results, it is clear that CT under heating considerably increases RHS. CT under heating considerably increases RHS. This increase in RHS may be due to acidification or germination-like phenomena during CT.",
author = "Seiji Noma and Kazuki Kiyohara and Rina Hirokado and Nami Yamashita and Yuya Migita and Motoharu Tanaka and Soichi Furukawa and Hirokazu Ogihara and Yasushi Morinaga and Noriyuki Igura and Mitsuya Shimoda",
year = "2018",
month = "3",
day = "1",
doi = "10.1016/j.jbiosc.2017.09.012",
language = "English",
volume = "125",
pages = "327--332",
journal = "Journal of Bioscience and Bioengineering",
issn = "1389-1723",
publisher = "The Society for Biotechnology, Japan",
number = "3",

}

TY - JOUR

T1 - Increase in hydrophobicity of Bacillus subtilis spores by heat, hydrostatic pressure, and pressurized carbon dioxide treatments

AU - Noma, Seiji

AU - Kiyohara, Kazuki

AU - Hirokado, Rina

AU - Yamashita, Nami

AU - Migita, Yuya

AU - Tanaka, Motoharu

AU - Furukawa, Soichi

AU - Ogihara, Hirokazu

AU - Morinaga, Yasushi

AU - Igura, Noriyuki

AU - Shimoda, Mitsuya

PY - 2018/3/1

Y1 - 2018/3/1

N2 - The effects of heat treatment (HT), hydrostatic pressure treatment (HPT), and pressurized carbon dioxide treatment (CT) on surface hydrophobicity of B. subtilis 168 spores were investigated. The spore surface hydrophobicity was measured by determining the ratio of hydrophobic spores (RHS) that were partitioned into the n-hexadecane phase from the aqueous spore suspension. The RHS after HT generally increased in a temperature-dependent manner and reached approximately 10% at temperatures above 60°C. The effects of pressurization by HPT and accompanying temperature on increased RHS were complex. The highest RHS after HPT was approximately 17%. Following CT, RHS reached approximately 80% at 5 MPa at 80°C for 30 min. An increased treatment temperature enhanced RHS by CT. The increase in RHS by CT led to the formation of spore clumps and adhesion of spores to hydrophobic surfaces. Acidification of spore suspension to pH 3.2, expected pH during CT, by HCl also increased the adhesion of spores at the similar degree with CT. The spore surface zeta potential distribution was not changed by CT. Furthermore, spores with increased RHS after CT had germination-like phenomena including loss of their refractility and enhanced staining by 4′,6-diamidino-2-phenylindole. Physiological germination that was induced by the addition of L-alanine also increased the RHS. From these results, it is clear that CT under heating considerably increases RHS. CT under heating considerably increases RHS. This increase in RHS may be due to acidification or germination-like phenomena during CT.

AB - The effects of heat treatment (HT), hydrostatic pressure treatment (HPT), and pressurized carbon dioxide treatment (CT) on surface hydrophobicity of B. subtilis 168 spores were investigated. The spore surface hydrophobicity was measured by determining the ratio of hydrophobic spores (RHS) that were partitioned into the n-hexadecane phase from the aqueous spore suspension. The RHS after HT generally increased in a temperature-dependent manner and reached approximately 10% at temperatures above 60°C. The effects of pressurization by HPT and accompanying temperature on increased RHS were complex. The highest RHS after HPT was approximately 17%. Following CT, RHS reached approximately 80% at 5 MPa at 80°C for 30 min. An increased treatment temperature enhanced RHS by CT. The increase in RHS by CT led to the formation of spore clumps and adhesion of spores to hydrophobic surfaces. Acidification of spore suspension to pH 3.2, expected pH during CT, by HCl also increased the adhesion of spores at the similar degree with CT. The spore surface zeta potential distribution was not changed by CT. Furthermore, spores with increased RHS after CT had germination-like phenomena including loss of their refractility and enhanced staining by 4′,6-diamidino-2-phenylindole. Physiological germination that was induced by the addition of L-alanine also increased the RHS. From these results, it is clear that CT under heating considerably increases RHS. CT under heating considerably increases RHS. This increase in RHS may be due to acidification or germination-like phenomena during CT.

UR - http://www.scopus.com/inward/record.url?scp=85032330696&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85032330696&partnerID=8YFLogxK

U2 - 10.1016/j.jbiosc.2017.09.012

DO - 10.1016/j.jbiosc.2017.09.012

M3 - Article

C2 - 29107628

AN - SCOPUS:85032330696

VL - 125

SP - 327

EP - 332

JO - Journal of Bioscience and Bioengineering

JF - Journal of Bioscience and Bioengineering

SN - 1389-1723

IS - 3

ER -