Increase of bone morphogenetic protein-7 expressing pulmonary resident cells in pneumonectomized rats

Taro Ohba, Hironobu Wada, Ichiro Yoshino, Shigetoshi Yoshida, Tetsuzo Tagawa, Fumihiro Shoji, Koji Yamazaki, Yoshihiko Maehara

研究成果: Contribution to journalArticle

抜粋

Purpose: Compensatory lung growth (CLG) is recognized in rodents subjected to major pulmonary resection; however, the source of cells constituting regenerated tissues during the CLG is still unknown. We investigated the differentiation of lung resident cells and the participation of bone marrow (BM)-derived cells in the remnant lung of pneumonectomized rats. Methods: After left pneumonectomy, the right remnant lung of Wistar rats was subjected to morphologic and molecular experiments at several time points. We studied the expression of bone morphogenic protein 7 (BMP-7), an accelerator of epithelial differentiation, based on the gene expression profile data of the remnant lung. Next, we evaluated the presence of GFP-positive cells in the remnant lung of Wistar rats that had received BM transplantation from green fluorescent protein (GFP) gene-transgenic Wistar rats prior to left pneumonectomy. Results: We observed progression of emphysematous change, modulation of gene expression profile, and proliferating cellular nuclear antigen-positive cells in the alveoli of the remnant lungs. BMP-7 protein positive cells were detected in the alveolar septa, which increased significantly over time with the progression of emphysematous change. No bone marrow-derived cells were detected in the right remnant lung of the GFP-BM transferred rats by fluorescence microscopy, immunohistochemistry, or polymerase chain reaction at any time. Conclusion: Lung resident cells appear to contribute to CLG, possibly via a trans- differentiation pathway.

元の言語英語
ページ(範囲)324-331
ページ数8
ジャーナルSurgery today
44
発行部数2
DOI
出版物ステータス出版済み - 2 1 2014

All Science Journal Classification (ASJC) codes

  • Surgery

フィンガープリント Increase of bone morphogenetic protein-7 expressing pulmonary resident cells in pneumonectomized rats' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用

    Ohba, T., Wada, H., Yoshino, I., Yoshida, S., Tagawa, T., Shoji, F., Yamazaki, K., & Maehara, Y. (2014). Increase of bone morphogenetic protein-7 expressing pulmonary resident cells in pneumonectomized rats. Surgery today, 44(2), 324-331. https://doi.org/10.1007/s00595-013-0604-7