Increased hepatic expression of dipeptidyl peptidase-4 in non-alcoholic fatty liver disease and its association with insulin resistance and glucose metabolism

Masayuki Miyazaki, Masaki Kato, Kosuke Tanaka, Masatake Tanaka, Motoyuki Kohjima, Kazuhiko Nakamura, Munechika Enjoji, Makoto Nakamuta, Kazuhiro Kotoh, Ryoichi Takayanagi

研究成果: ジャーナルへの寄稿記事

63 引用 (Scopus)

抄録

Dipeptidyl peptidase-4 (DPP4) is a serine protease that degrades glucagon-like peptide-1 (GLP-1), an incretin hormone that stimulates insulin secretion from pancreatic β-cells. DPP4 is also involved in the regulation of T cell-mediated inflammatory processes. These properties of DPP4 suggest that it may play a role in the progression of non-alcoholic fatty liver disease (NAFLD). Hepatic DPP4 mRNA expression levels were analyzed by real-time PCR using liver biopsy samples from 17 NAFLD patients and 10 healthy subjects. In NAFLD patients, we also examined correlations between DPP4 expression levels and metabolic factors, including homeostasis model assessment-insulin resistance (HOMA-IR), body mass index (BMI), and serum cholesterol and triglyceride levels. To examine the potential effects of nutritional factors, DPP4 expression levels were analyzed in HepG2 cells subjected to various culture conditions. Hepatic DPP4 mRNA expression was significantly greater in NAFLD patients than in control subjects. DPP4 expression levels were negatively correlated with HOMA-IR and positively correlated with serum cholesterol levels. In HepG2 cells, high glucose significantly enhanced DPP4 expression, whereas insulin, fatty acids and cholesterol did not. Increased hepatic expression of DPP4 in NAFLD may be associated with metabolic factors, including insulin resistance, and may adversely affect glucose metabolism in this liver disease.

元の言語英語
ページ(範囲)729-733
ページ数5
ジャーナルMolecular medicine reports
5
発行部数3
DOI
出版物ステータス出版済み - 3 1 2012

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Dipeptidyl Peptidase 4
Metabolism
Liver
Insulin Resistance
Insulin
Glucose
Cholesterol
Hep G2 Cells
Homeostasis
Non-alcoholic Fatty Liver Disease
Incretins
Messenger RNA
Glucagon-Like Peptide 1
T-cells
Biopsy
Serine Proteases
Serum
Liver Diseases
Real-Time Polymerase Chain Reaction
Healthy Volunteers

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Oncology
  • Cancer Research

これを引用

Increased hepatic expression of dipeptidyl peptidase-4 in non-alcoholic fatty liver disease and its association with insulin resistance and glucose metabolism. / Miyazaki, Masayuki; Kato, Masaki; Tanaka, Kosuke; Tanaka, Masatake; Kohjima, Motoyuki; Nakamura, Kazuhiko; Enjoji, Munechika; Nakamuta, Makoto; Kotoh, Kazuhiro; Takayanagi, Ryoichi.

:: Molecular medicine reports, 巻 5, 番号 3, 01.03.2012, p. 729-733.

研究成果: ジャーナルへの寄稿記事

Miyazaki, Masayuki ; Kato, Masaki ; Tanaka, Kosuke ; Tanaka, Masatake ; Kohjima, Motoyuki ; Nakamura, Kazuhiko ; Enjoji, Munechika ; Nakamuta, Makoto ; Kotoh, Kazuhiro ; Takayanagi, Ryoichi. / Increased hepatic expression of dipeptidyl peptidase-4 in non-alcoholic fatty liver disease and its association with insulin resistance and glucose metabolism. :: Molecular medicine reports. 2012 ; 巻 5, 番号 3. pp. 729-733.
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abstract = "Dipeptidyl peptidase-4 (DPP4) is a serine protease that degrades glucagon-like peptide-1 (GLP-1), an incretin hormone that stimulates insulin secretion from pancreatic β-cells. DPP4 is also involved in the regulation of T cell-mediated inflammatory processes. These properties of DPP4 suggest that it may play a role in the progression of non-alcoholic fatty liver disease (NAFLD). Hepatic DPP4 mRNA expression levels were analyzed by real-time PCR using liver biopsy samples from 17 NAFLD patients and 10 healthy subjects. In NAFLD patients, we also examined correlations between DPP4 expression levels and metabolic factors, including homeostasis model assessment-insulin resistance (HOMA-IR), body mass index (BMI), and serum cholesterol and triglyceride levels. To examine the potential effects of nutritional factors, DPP4 expression levels were analyzed in HepG2 cells subjected to various culture conditions. Hepatic DPP4 mRNA expression was significantly greater in NAFLD patients than in control subjects. DPP4 expression levels were negatively correlated with HOMA-IR and positively correlated with serum cholesterol levels. In HepG2 cells, high glucose significantly enhanced DPP4 expression, whereas insulin, fatty acids and cholesterol did not. Increased hepatic expression of DPP4 in NAFLD may be associated with metabolic factors, including insulin resistance, and may adversely affect glucose metabolism in this liver disease.",
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