Increasing Elasticity through Changes in the Secondary Structure of Gelatin by Gelation in a Microsized Lipid Space

Atsushi Sakai, Yoshihiro Murayama, Kei Fujiwara, Takahiro Fujisawa, Saori Sasaki, Satoru Kidoaki, Miho Yanagisawa

研究成果: ジャーナルへの寄稿記事

4 引用 (Scopus)

抄録

Even though microgels are used in a wide variety of applications, determining their mechanical properties has been elusive because of the difficulties in analysis. In this study, we investigated the surface elasticity of a spherical microgel of gelatin prepared inside a lipid droplet by using micropipet aspiration. We found that gelation inside a microdroplet covered with lipid membranes increased Young's modulus E toward a plateau value E∗ along with a decrease in gel size. In the case of 5.0 wt % gelatin gelled inside a microsized lipid space, the E∗ for small microgels with R ≤ 50 μm was 10-fold higher (35-39 kPa) than that for the bulk gel (∼3 kPa). Structural analysis using circular dichroism spectroscopy and a fluorescence indicator for ordered beta sheets demonstrated that the smaller microgels contained more beta sheets in the structure than the bulk gel. Our finding indicates that the confinement size of gelling polymers becomes a factor in the variation of elasticity of protein-based microgels via secondary structure changes.

元の言語英語
ページ(範囲)477-483
ページ数7
ジャーナルACS Central Science
4
発行部数4
DOI
出版物ステータス出版済み - 4 25 2018

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Gelation
Gelatin
Lipids
Elasticity
Gels
Circular dichroism spectroscopy
Membrane Lipids
Structural analysis
Polymers
Elastic moduli
Fluorescence
Proteins
Mechanical properties

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

これを引用

Increasing Elasticity through Changes in the Secondary Structure of Gelatin by Gelation in a Microsized Lipid Space. / Sakai, Atsushi; Murayama, Yoshihiro; Fujiwara, Kei; Fujisawa, Takahiro; Sasaki, Saori; Kidoaki, Satoru; Yanagisawa, Miho.

:: ACS Central Science, 巻 4, 番号 4, 25.04.2018, p. 477-483.

研究成果: ジャーナルへの寄稿記事

Sakai, Atsushi ; Murayama, Yoshihiro ; Fujiwara, Kei ; Fujisawa, Takahiro ; Sasaki, Saori ; Kidoaki, Satoru ; Yanagisawa, Miho. / Increasing Elasticity through Changes in the Secondary Structure of Gelatin by Gelation in a Microsized Lipid Space. :: ACS Central Science. 2018 ; 巻 4, 番号 4. pp. 477-483.
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