Inducible production of erythropoietin using new runaway-type vector by mammalian cell culture in spinner flask

Hideyo Kirinaka, Masamichi Kamihira, Takeshi Kobayashi, Shinji Iijima

研究成果: Contribution to journalArticle査読

2 被引用数 (Scopus)

抄録

Human erythropoietin (HuEPO) gene was inserted into an SV40-based episomal vector with a temperature-sensitive large T antigen which enabled uncontrolled plasmid replication and inducible HuEPO production at temperatures below 33°C. Good productivity of HuEPO was obtained when temperature was lowered to 30°C at relatively higher cell density in Petri dish culture. Therefore, we cultured the cells immobilized on microcarriers in a spinner flask at 37°C until the cell density reached 1.8 × 104 cells/cm2-microcarrier surface area, and then the temperature was lowered to 30°C for HuEPO production. Using this process, HuEPO productivity was enhanced 7-18 times that of the Petri dish cultures, and a high concentration of HuEPO was accumulated in the medium.

本文言語英語
ページ(範囲)483-485
ページ数3
ジャーナルJournal of Fermentation and Bioengineering
78
6
DOI
出版ステータス出版済み - 1994
外部発表はい

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 応用微生物学とバイオテクノロジー

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