TY - JOUR
T1 - Inducible production of erythropoietin using new runaway-type vector by mammalian cell culture in spinner flask
AU - Kirinaka, Hideyo
AU - Kamihira, Masamichi
AU - Kobayashi, Takeshi
AU - Iijima, Shinji
PY - 1994
Y1 - 1994
N2 - Human erythropoietin (HuEPO) gene was inserted into an SV40-based episomal vector with a temperature-sensitive large T antigen which enabled uncontrolled plasmid replication and inducible HuEPO production at temperatures below 33°C. Good productivity of HuEPO was obtained when temperature was lowered to 30°C at relatively higher cell density in Petri dish culture. Therefore, we cultured the cells immobilized on microcarriers in a spinner flask at 37°C until the cell density reached 1.8 × 104 cells/cm2-microcarrier surface area, and then the temperature was lowered to 30°C for HuEPO production. Using this process, HuEPO productivity was enhanced 7-18 times that of the Petri dish cultures, and a high concentration of HuEPO was accumulated in the medium.
AB - Human erythropoietin (HuEPO) gene was inserted into an SV40-based episomal vector with a temperature-sensitive large T antigen which enabled uncontrolled plasmid replication and inducible HuEPO production at temperatures below 33°C. Good productivity of HuEPO was obtained when temperature was lowered to 30°C at relatively higher cell density in Petri dish culture. Therefore, we cultured the cells immobilized on microcarriers in a spinner flask at 37°C until the cell density reached 1.8 × 104 cells/cm2-microcarrier surface area, and then the temperature was lowered to 30°C for HuEPO production. Using this process, HuEPO productivity was enhanced 7-18 times that of the Petri dish cultures, and a high concentration of HuEPO was accumulated in the medium.
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U2 - 10.1016/0922-338X(94)90053-1
DO - 10.1016/0922-338X(94)90053-1
M3 - Article
AN - SCOPUS:0028607303
VL - 78
SP - 483
EP - 485
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
SN - 1389-1723
IS - 6
ER -