Induction and suppression of contact sensitivity to fluorescein isothiocyanate (FITC)

Masutaka Furue, K. Tamaki

研究成果: ジャーナルへの寄稿記事

11 引用 (Scopus)

抄録

Although both fluorescein isothiocyanate (FITC) and trinitrophenyl (TNP) covalently couple primarily to the ε-amino group of lysine residues of surface glycoprotein, FITC is structurally different from TNP. In this paper, we investigated the immune regulatory mechanisms in contact sensitivity (CS) to FITC by the administration of FITC and FITC-conjugated epidermal cells (FITC-EC) via various routes. Mice injected with FITC via i.p. or i.v. route did not induce CS but induced hyporesponsiveness to the following sensitization with FITC painting. Mice injected with FITC via s.c. route induced neither CS nor hyporesponsiveness to the following FITC painting. Administration of FITC via i.v. route was demonstrated to induce hapten-specific suppressor T cells. Inoculation of FITC-EC via s.c. or i.p. route induced CS, whereas injection of FITC-EC via i.v. route did not induce CS but induced hyporesponsiveness to the following FITC painting. The results are compared with the previous data obtained by trinitrobenzene sulfonate and trinitrophenyl-conjugated epidermal cells.

元の言語英語
ページ(範囲)139-142
ページ数4
ジャーナルJournal of Investigative Dermatology
85
発行部数2
DOI
出版物ステータス出版済み - 1 1 1985
外部発表Yes

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Contact Dermatitis
Fluorescein
Paintings
Painting
isothiocyanic acid
Trinitrobenzenesulfonic Acid
T-cells
Haptens
Membrane Glycoproteins
Lysine

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

これを引用

Induction and suppression of contact sensitivity to fluorescein isothiocyanate (FITC). / Furue, Masutaka; Tamaki, K.

:: Journal of Investigative Dermatology, 巻 85, 番号 2, 01.01.1985, p. 139-142.

研究成果: ジャーナルへの寄稿記事

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abstract = "Although both fluorescein isothiocyanate (FITC) and trinitrophenyl (TNP) covalently couple primarily to the ε-amino group of lysine residues of surface glycoprotein, FITC is structurally different from TNP. In this paper, we investigated the immune regulatory mechanisms in contact sensitivity (CS) to FITC by the administration of FITC and FITC-conjugated epidermal cells (FITC-EC) via various routes. Mice injected with FITC via i.p. or i.v. route did not induce CS but induced hyporesponsiveness to the following sensitization with FITC painting. Mice injected with FITC via s.c. route induced neither CS nor hyporesponsiveness to the following FITC painting. Administration of FITC via i.v. route was demonstrated to induce hapten-specific suppressor T cells. Inoculation of FITC-EC via s.c. or i.p. route induced CS, whereas injection of FITC-EC via i.v. route did not induce CS but induced hyporesponsiveness to the following FITC painting. The results are compared with the previous data obtained by trinitrobenzene sulfonate and trinitrophenyl-conjugated epidermal cells.",
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