Induction of broadly neutralizing antibodies against measles virus mutants using a polyepitope vaccine strategy

Fabienne B. Bouche, André Steinmetz, Yusuke Yanagi, Claude P. Muller

研究成果: Contribution to journalArticle査読

21 被引用数 (Scopus)

抄録

Chimeric molecules expressing multiple copies of the loop-forming hemagglutinin noose epitope (designated as "L"; aa386-400), a protective B cell epitope of the measles virus were generated by recombinant technology. The recombinant polyepitope [L4T4]2 combining two sets of four repeats of the L epitope and with two sets of four repeats of the human promiscuous T cell epitope of tetanus toxoid ("T", tt830-844) was produced in transgenic carrot plants. After intraperitoneal immunization of mice with plant membrane extract, sera neutralized all wild-type viruses. In a modified plaque reduction neutralization assay based on CD150-transfected Vero cells anti-[L4T 4]2 sera neutralized all field isolates, irrespective of mutations in the L epitope. Even viruses with a mutation in the contact residues of a neutralizing L-specific monoclonal antibody or two mutations in other positions of the epitope were equally sensitive to neutralization. These results suggest that the multiple copies of the L epitope fold into different conformations that induce a repertoire of B cells diverse enough to overcome the genetic diversity of field viruses.

本文言語英語
ページ(範囲)2074-2077
ページ数4
ジャーナルVaccine
23
17-18
DOI
出版ステータス出版済み - 3 18 2005

All Science Journal Classification (ASJC) codes

  • 分子医療
  • 免疫学および微生物学(全般)
  • 獣医学(全般)
  • 公衆衛生学、環境および労働衛生
  • 感染症

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