Influence of cluster formation of acidic phospholipids on decrease in the affinity for ATP of DnaA protein

Tohru Mizushima, Yuichi Ishikawa, Eishi Obana, Masakazu Hase, Toshio Kubota, Tsutomu Katayama, Toyoki Kunitake, Eiji Watanabe, Kazuhisa Sekimizu

研究成果: Contribution to journalArticle査読

49 被引用数 (Scopus)

抄録

DnaA protein is the initiator of chromosomal DNA replication in Escherichia coli. We examined the influence of artificial mixed membrane composed of synthetic acidic (phosphate) lipid and basic (ammonium) lipid on the affinity of DnaA protein for ATP. Two sets of acidic and basic lipids with distinguishable numbers of hydrophobic alkyl chains were devised. Synthetic membranes made of the sole acidic lipid but not the basic bilayers inhibited the ATP binding to DnaA protein and stimulated the release of ATP from the ATP-DnaA complex. The basic bilayer-forming compounds served as the matrix for the guest acidic lipids. Acidic lipids dispersed in the basic matrix membrane had little effect on ATP binding and on ATP release. Conversely, acidic lipids forming cluster structures in the mixed artificial membranes inhibited the ATP binding and stimulated the release of ATP. These observations suggest that in mixed lipid bilayers, a cluster structure of acidic lipids seems to be an important parameter to decrease the affinity of DnaA protein for ATP.

本文言語英語
ページ(範囲)3633-3638
ページ数6
ジャーナルJournal of Biological Chemistry
271
7
DOI
出版ステータス出版済み - 2 16 1996

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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