Influence of graft interface polarity on hydration/dehydration of grafted thermoresponsive polymer brushes and steroid separation using all-aqueous chromatography

Kenichi Nagase, Jun Kobayashi, Akihiko Kikuchi, Yoshikatsu Akiyama, Masahiko Annaka, Hideko Kanazawa, Teruo Okano

研究成果: ジャーナルへの寄稿記事

51 引用 (Scopus)

抄録

We have prepared poly(N-isopropylacrylamide) (PIPAAm) brush-grafted surfaces with varied temperature-responsive hydrophobic properties through surface-initiated atom transfer radical polymerization (ATRP). These temperature-responsive surfaces were characterized by Chromatographic analysis using modified silica beads as a Chromatographic stationary phase in aqueous mobile phase. Mixed silane self-assembled monolayers (SAMs) comprising ATRP initiator and silanes with various terminal functional groups were formed on the silica bead surfaces. IPAAm was then polymerized by ATRP using the CuCl/CuCl2/Mc6TREN catalyst system in 2-propanol at 25°C for 16 h. The Chromatographic retention behavior of steroids on the resulting PIPAAm brushes made on more polar silane components was distinct from that on more apolar silane interfaces. Retention times for steroids on PIPAAm mixed apolar silane graft interfaces were significantly longer than those on analogous polar silane interfaces due to enhanced dehydration of PIPAAm brushes on apolar silane-grafted surfaces. Changes in retention factor, k′, on polar silane PIPAAm-grafted interfaces were relatively large compared to that on apolar PIPAAm grafted interfaces due to larger hydration/dehydration alterations of grafted PIPAAm brushes on the former surfaces. Applied step-temperature gradients from 50 to 10 °C show that PIPAAm brushes on polar silane interfaces tend to hydrate more, leading to shorter retention times. In conclusion, the polarity of the grafted interface significantly influences the grafted PIPAAm brush hydration/dehydration characteristics and subsequently also the temperature-modulated separation of bioactive compounds in all-aqueous chromatography.

元の言語英語
ページ(範囲)10981-10987
ページ数7
ジャーナルLangmuir
24
発行部数19
DOI
出版物ステータス出版済み - 10 7 2008

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Silanes
steroids
brushes
Brushes
chromatography
Chromatography
Dehydration
Grafts
silanes
dehydration
Hydration
hydration
polarity
Polymers
Steroids
polymers
Atom transfer radical polymerization
polymerization
Silicon Dioxide
beads

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

これを引用

Influence of graft interface polarity on hydration/dehydration of grafted thermoresponsive polymer brushes and steroid separation using all-aqueous chromatography. / Nagase, Kenichi; Kobayashi, Jun; Kikuchi, Akihiko; Akiyama, Yoshikatsu; Annaka, Masahiko; Kanazawa, Hideko; Okano, Teruo.

:: Langmuir, 巻 24, 番号 19, 07.10.2008, p. 10981-10987.

研究成果: ジャーナルへの寄稿記事

Nagase, Kenichi ; Kobayashi, Jun ; Kikuchi, Akihiko ; Akiyama, Yoshikatsu ; Annaka, Masahiko ; Kanazawa, Hideko ; Okano, Teruo. / Influence of graft interface polarity on hydration/dehydration of grafted thermoresponsive polymer brushes and steroid separation using all-aqueous chromatography. :: Langmuir. 2008 ; 巻 24, 番号 19. pp. 10981-10987.
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abstract = "We have prepared poly(N-isopropylacrylamide) (PIPAAm) brush-grafted surfaces with varied temperature-responsive hydrophobic properties through surface-initiated atom transfer radical polymerization (ATRP). These temperature-responsive surfaces were characterized by Chromatographic analysis using modified silica beads as a Chromatographic stationary phase in aqueous mobile phase. Mixed silane self-assembled monolayers (SAMs) comprising ATRP initiator and silanes with various terminal functional groups were formed on the silica bead surfaces. IPAAm was then polymerized by ATRP using the CuCl/CuCl2/Mc6TREN catalyst system in 2-propanol at 25°C for 16 h. The Chromatographic retention behavior of steroids on the resulting PIPAAm brushes made on more polar silane components was distinct from that on more apolar silane interfaces. Retention times for steroids on PIPAAm mixed apolar silane graft interfaces were significantly longer than those on analogous polar silane interfaces due to enhanced dehydration of PIPAAm brushes on apolar silane-grafted surfaces. Changes in retention factor, k′, on polar silane PIPAAm-grafted interfaces were relatively large compared to that on apolar PIPAAm grafted interfaces due to larger hydration/dehydration alterations of grafted PIPAAm brushes on the former surfaces. Applied step-temperature gradients from 50 to 10 °C show that PIPAAm brushes on polar silane interfaces tend to hydrate more, leading to shorter retention times. In conclusion, the polarity of the grafted interface significantly influences the grafted PIPAAm brush hydration/dehydration characteristics and subsequently also the temperature-modulated separation of bioactive compounds in all-aqueous chromatography.",
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AU - Akiyama, Yoshikatsu

AU - Annaka, Masahiko

AU - Kanazawa, Hideko

AU - Okano, Teruo

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N2 - We have prepared poly(N-isopropylacrylamide) (PIPAAm) brush-grafted surfaces with varied temperature-responsive hydrophobic properties through surface-initiated atom transfer radical polymerization (ATRP). These temperature-responsive surfaces were characterized by Chromatographic analysis using modified silica beads as a Chromatographic stationary phase in aqueous mobile phase. Mixed silane self-assembled monolayers (SAMs) comprising ATRP initiator and silanes with various terminal functional groups were formed on the silica bead surfaces. IPAAm was then polymerized by ATRP using the CuCl/CuCl2/Mc6TREN catalyst system in 2-propanol at 25°C for 16 h. The Chromatographic retention behavior of steroids on the resulting PIPAAm brushes made on more polar silane components was distinct from that on more apolar silane interfaces. Retention times for steroids on PIPAAm mixed apolar silane graft interfaces were significantly longer than those on analogous polar silane interfaces due to enhanced dehydration of PIPAAm brushes on apolar silane-grafted surfaces. Changes in retention factor, k′, on polar silane PIPAAm-grafted interfaces were relatively large compared to that on apolar PIPAAm grafted interfaces due to larger hydration/dehydration alterations of grafted PIPAAm brushes on the former surfaces. Applied step-temperature gradients from 50 to 10 °C show that PIPAAm brushes on polar silane interfaces tend to hydrate more, leading to shorter retention times. In conclusion, the polarity of the grafted interface significantly influences the grafted PIPAAm brush hydration/dehydration characteristics and subsequently also the temperature-modulated separation of bioactive compounds in all-aqueous chromatography.

AB - We have prepared poly(N-isopropylacrylamide) (PIPAAm) brush-grafted surfaces with varied temperature-responsive hydrophobic properties through surface-initiated atom transfer radical polymerization (ATRP). These temperature-responsive surfaces were characterized by Chromatographic analysis using modified silica beads as a Chromatographic stationary phase in aqueous mobile phase. Mixed silane self-assembled monolayers (SAMs) comprising ATRP initiator and silanes with various terminal functional groups were formed on the silica bead surfaces. IPAAm was then polymerized by ATRP using the CuCl/CuCl2/Mc6TREN catalyst system in 2-propanol at 25°C for 16 h. The Chromatographic retention behavior of steroids on the resulting PIPAAm brushes made on more polar silane components was distinct from that on more apolar silane interfaces. Retention times for steroids on PIPAAm mixed apolar silane graft interfaces were significantly longer than those on analogous polar silane interfaces due to enhanced dehydration of PIPAAm brushes on apolar silane-grafted surfaces. Changes in retention factor, k′, on polar silane PIPAAm-grafted interfaces were relatively large compared to that on apolar PIPAAm grafted interfaces due to larger hydration/dehydration alterations of grafted PIPAAm brushes on the former surfaces. Applied step-temperature gradients from 50 to 10 °C show that PIPAAm brushes on polar silane interfaces tend to hydrate more, leading to shorter retention times. In conclusion, the polarity of the grafted interface significantly influences the grafted PIPAAm brush hydration/dehydration characteristics and subsequently also the temperature-modulated separation of bioactive compounds in all-aqueous chromatography.

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