Influence of N-methyl-N-nitrosourea treatment on embryogenesis of Bombyx mori

Yutaka Kawaguchi, Takahiro Kusakabe, Katsumi Koga

研究成果: ジャーナルへの寄稿記事

抄録

Embryonic malformation was induced by immersing Bombyx mori eggs at 3.5 h after oviposition in a 10 mM N-methyl-N-nitrosourea (MNU) solution for 15 min. About 80% of thus treated eggs could not hatch. When observed under a light microscope using unsectioned transparent specimens of unhatched eggs, the embryos exhibited abnormalities such as the absence of inner organs, twisting body axis, incomplete body segments and anomalous segmentation. These findings confirmed that the immersing method worked well and MNU strongly provokes various forms of somatic impairment in developing embryos.

元の言語英語
ページ(範囲)59-64
ページ数6
ジャーナルJournal of the Faculty of Agriculture, Kyushu University
48
発行部数1-2
出版物ステータス出版済み - 10 1 2003

Fingerprint

N-methyl-N-nitrosourea
Methylnitrosourea
Bombyx
Bombyx mori
Eggs
Embryonic Development
embryo (animal)
embryogenesis
light microscopes
Embryonic Structures
Oviposition
oviposition
Light
methodology

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

これを引用

Influence of N-methyl-N-nitrosourea treatment on embryogenesis of Bombyx mori. / Kawaguchi, Yutaka; Kusakabe, Takahiro; Koga, Katsumi.

:: Journal of the Faculty of Agriculture, Kyushu University, 巻 48, 番号 1-2, 01.10.2003, p. 59-64.

研究成果: ジャーナルへの寄稿記事

@article{08f9e53a6c784d0bafd7ae73a364cabb,
title = "Influence of N-methyl-N-nitrosourea treatment on embryogenesis of Bombyx mori",
abstract = "Embryonic malformation was induced by immersing Bombyx mori eggs at 3.5 h after oviposition in a 10 mM N-methyl-N-nitrosourea (MNU) solution for 15 min. About 80{\%} of thus treated eggs could not hatch. When observed under a light microscope using unsectioned transparent specimens of unhatched eggs, the embryos exhibited abnormalities such as the absence of inner organs, twisting body axis, incomplete body segments and anomalous segmentation. These findings confirmed that the immersing method worked well and MNU strongly provokes various forms of somatic impairment in developing embryos.",
author = "Yutaka Kawaguchi and Takahiro Kusakabe and Katsumi Koga",
year = "2003",
month = "10",
day = "1",
language = "English",
volume = "48",
pages = "59--64",
journal = "Journal of the Faculty of Agriculture, Kyushu University",
issn = "0023-6152",
publisher = "Faculty of Agriculture, Kyushu University",
number = "1-2",

}

TY - JOUR

T1 - Influence of N-methyl-N-nitrosourea treatment on embryogenesis of Bombyx mori

AU - Kawaguchi, Yutaka

AU - Kusakabe, Takahiro

AU - Koga, Katsumi

PY - 2003/10/1

Y1 - 2003/10/1

N2 - Embryonic malformation was induced by immersing Bombyx mori eggs at 3.5 h after oviposition in a 10 mM N-methyl-N-nitrosourea (MNU) solution for 15 min. About 80% of thus treated eggs could not hatch. When observed under a light microscope using unsectioned transparent specimens of unhatched eggs, the embryos exhibited abnormalities such as the absence of inner organs, twisting body axis, incomplete body segments and anomalous segmentation. These findings confirmed that the immersing method worked well and MNU strongly provokes various forms of somatic impairment in developing embryos.

AB - Embryonic malformation was induced by immersing Bombyx mori eggs at 3.5 h after oviposition in a 10 mM N-methyl-N-nitrosourea (MNU) solution for 15 min. About 80% of thus treated eggs could not hatch. When observed under a light microscope using unsectioned transparent specimens of unhatched eggs, the embryos exhibited abnormalities such as the absence of inner organs, twisting body axis, incomplete body segments and anomalous segmentation. These findings confirmed that the immersing method worked well and MNU strongly provokes various forms of somatic impairment in developing embryos.

UR - http://www.scopus.com/inward/record.url?scp=1642477590&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1642477590&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:1642477590

VL - 48

SP - 59

EP - 64

JO - Journal of the Faculty of Agriculture, Kyushu University

JF - Journal of the Faculty of Agriculture, Kyushu University

SN - 0023-6152

IS - 1-2

ER -