Influence of reactive species on the modification of biomolecules generated from the soft plasma

Pankaj Attri, Naresh Kumar, Ji Hoon Park, Dharmendra Kumar Yadav, Sooho Choi, Han S. Uhm, In Tae Kim, Eun Ha Choi, Weontae Lee

研究成果: Contribution to journalArticle査読

66 被引用数 (Scopus)

抄録

Plasma medicine is an upcoming research area that has attracted the scientists to explore more deeply the utility of plasma. So, apart from the treating biomaterials and tissues with plasma, we have studied the effect of soft plasma with different feeding gases such as Air, N2 and Ar on modification of biomolecules. Hence, in this work we have used the soft plasma on biomolecules such as proteins ((Hemoglobin (Hb) and Myoglobin (Mb)), calf thymus DNA and amino acids. The structural changes or structural modification of proteins and DNA have been studied using circular dichroism (CD), fluorescence spectroscopy, protein oxidation test, gel electrophoresis, UV-vis spectroscopy, dynamic light scattering (DLS) and 1D NMR, while Liquid Chromatograph/Capillary Electrophoresis-Mass Spectrometer (LC/CE-MS) based on qualitative and quantitative bio-analysis have been used to study the modification of amino acids. Further, the thermal analysis of the protein has been studied with differential scanning calorimetry (DSC) and CD. Additionally, we have performed docking studies of H2O2 with Hb and Mb, which reveals that H2O2 molecules preferably attack the amino acids near heme group. We have also shown that N2 gas plasma has strong deformation action on biomolecules and compared to other gases plasma.

本文言語英語
論文番号8221
ジャーナルScientific reports
5
DOI
出版ステータス出版済み - 2 4 2015
外部発表はい

All Science Journal Classification (ASJC) codes

  • General

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