Influence of single-nucleotide polymorphisms in the gene encoding granule-bound starch synthase I on amylose content in Vietnamese rice cultivars

Tran Thi Thu Hoai, Hiroaki Matsusaka, Yoshiko Toyosawa, Tran Danh Suu, Hikaru Satoh, Toshihiro Kumamaru

研究成果: ジャーナルへの寄稿記事

7 引用 (Scopus)

抄録

Amylose content is one of the most important factors influencing the physical and chemical properties of starch in rice. Analysis of 352 Vietnamese rice cultivars revealed a wide range of variation in apparent amylose content and the expression level of granule-bound starch synthase. On the basis of single-nucleotide polymorphisms (SNP) at the splicing donor site of the first intron and in the coding region of the granule-bound starch synthase I gene, Waxy gene, alleles can be classified into seven groups that reflect differences in apparent amylose content. The very low and low apparent amylose content levels were tightly associated with a G to T in the first intron whereas intermediate and high amylose was associated with a T genotype at SNP in exon 10. The correlation between the combination of T genotype at SNP in the first intron, C in exon 6, or C in exon 10 was predominant among low amylose rice varieties. Our analysis confirmed the existence of Wxop allele in Vietnamese rice germplasm. The results of this study suggest that the low amylose properties of Vietnamese local rice germplasm are attributable to spontaneous mutations at exons, and not at the splicing donor site.

元の言語英語
ページ(範囲)142-148
ページ数7
ジャーナルBreeding Science
64
発行部数2
DOI
出版物ステータス出版済み - 1 1 2014

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starch synthase
Amylose
amylose
single nucleotide polymorphism
Single Nucleotide Polymorphism
granules
rice
cultivars
exons
Exons
Genes
Introns
genes
introns
germplasm
Starch Synthase
Alleles
Genotype
alleles
rice starch

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Agronomy and Crop Science
  • Genetics

これを引用

Influence of single-nucleotide polymorphisms in the gene encoding granule-bound starch synthase I on amylose content in Vietnamese rice cultivars. / Hoai, Tran Thi Thu; Matsusaka, Hiroaki; Toyosawa, Yoshiko; Suu, Tran Danh; Satoh, Hikaru; Kumamaru, Toshihiro.

:: Breeding Science, 巻 64, 番号 2, 01.01.2014, p. 142-148.

研究成果: ジャーナルへの寄稿記事

Hoai, Tran Thi Thu ; Matsusaka, Hiroaki ; Toyosawa, Yoshiko ; Suu, Tran Danh ; Satoh, Hikaru ; Kumamaru, Toshihiro. / Influence of single-nucleotide polymorphisms in the gene encoding granule-bound starch synthase I on amylose content in Vietnamese rice cultivars. :: Breeding Science. 2014 ; 巻 64, 番号 2. pp. 142-148.
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