Influences of vascular niches on hematopoietic stem cell fate

Yuya Kunisaki, Paul S. Frenette

研究成果: ジャーナルへの寄稿記事

18 引用 (Scopus)

抄録

The fate decision of hematopoietic stem cells (HSCs), quiescence, proliferation or differentiation, is uniquely determined by functionally specialized microenvironments defined as the HSC niches. However, whether quiescence and proliferation of HSCs are regulated by spatially distinct niches is unclear. Although various candidate stromal cells have been identified as potential niche cells, the spatial localization of quiescent HSCs in the bone marrow remains controversial. In our recent study, we have established whole-mount confocal immunofluorescence techniques, which allow us to precisely assess the localization of HSCs and their relationships with stromal structures. Furthermore, we have assessed the significance of these associations using a computational simulation. These novel analyses have revealed that quiescent HSCs are specifically associated with small caliber arterioles, which are predominantly distributed in the endosteal bone marrow while the associations with sinusoidal vessels or osteoblasts are not significant. Physical ablation of the arteriolar niche causes the shift of HSC localization to sinusoidal niches, where HSCs are switched into non-quiescent status. This new imaging analyses together with previous studies suggest the presence of spatially distinct vascular niches for quiescent and non-quiescent (proliferating) HSCs in the bone marrow.

元の言語英語
ページ(範囲)699-705
ページ数7
ジャーナルInternational journal of hematology
99
発行部数6
DOI
出版物ステータス出版済み - 6 2014

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Hematopoietic Stem Cells
Blood Vessels
Bone Marrow
Stem Cell Niche
Arterioles
Stromal Cells
Osteoblasts
Fluorescent Antibody Technique
Cell Differentiation
Cell Proliferation

All Science Journal Classification (ASJC) codes

  • Hematology

これを引用

Influences of vascular niches on hematopoietic stem cell fate. / Kunisaki, Yuya; Frenette, Paul S.

:: International journal of hematology, 巻 99, 番号 6, 06.2014, p. 699-705.

研究成果: ジャーナルへの寄稿記事

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