Inhibition mode of soybean lipoxygenase-1 by quercetin

Tae Joung Ha, Kuniyoshi Shimizu, Tetsuya Ogura, Isao Kubo

研究成果: ジャーナルへの寄稿学術誌査読

11 被引用数 (Scopus)

抄録

Quercetin noncompetitively inhibited the peroxidation of linoleic acid catalyzed by soybean lipoxygenase-1 (EC 1.13.11.12, Type 1) with an IC 50 value of 4.8 mm (1.45 μg/ml). This inhibition is considered to proceed in sequential order, by initially reducing the ferric form of the enzyme to an inactive ferrous form and then, by chelating the iron of the active site of the enzyme. In the pseudoperoxidase assay, quercetin was slowly oxidized by hydroperoxides to a rather stable intermediate, 2-(3,4-dihydroxybenzoyl)-2,4,6- trihydroxybenzofuran-3(2H)-one, and this oxidized intermediate still inhibited the enzymatic oxidation, probably as a chelator. Rutin and kaempferol also exhibited lipoxygenase-1 inhibitory activity, but to a much lesser extent than quercetin.

本文言語英語
ページ(範囲)1893-1903
ページ数11
ジャーナルChemistry and Biodiversity
7
8
DOI
出版ステータス出版済み - 8月 2010
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • バイオエンジニアリング
  • 生化学
  • 化学 (全般)
  • 分子医療
  • 分子生物学

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