Inhibition of benzo[a]pyrene-induced mutagenesis by (-)-epigallocatechin gallate in the lung of rpsL transgenic mice

Shigeharu Muto, Tsuyoshi Yokoi, Yoichi Gondo, Motoya Katsuki, Yoshiyuki Shioyama, Ken Ichi Fujita, Tetsuya Kamataki

研究成果: ジャーナルへの寄稿記事

52 引用 (Scopus)

抄録

Epigallocatechin gallate (EGCG) is a major water-soluble component of green tea. The antimutagenic activity of EGCG against benzo[a]pyrene (B[a]P)-induced mutations was assessed by using transgenic mice carrying the rpsL gene as a monitor of mutations. Seven-week-old male mice were given drinking water containing EGCG for 3 weeks. On day 7, mice were treated with a single i.p. injection of B[a]P (500 mg/kg body wt). Two weeks after the injection, the mutations in the rpsL gene were analyzed. B[a]P treatment resulted in an ~ 4-fold increase of mutation frequency at the rpsL gene in the lung. An ~ 60% reduction in the B[a]P-induced mutations in the lung was observed when mice were given EGCG at concentrations > 0.005%. B[a]P-induced mutations mainly occurred at G:C base-pairs in the several specific nucleotide sequences of the rpsL gene. These were AGG, CGG, CGT, TGG, TGC and GGT: all of them contained a guanine residue. Mutations seen similarly in the human Ki-ras codon 12 or p53 codons 157, 248, and 273 of lung tumor were also found in the rpsL gene, and the mutations were suppressed by the EGCG treatment. In conclusion, the antimutagenic effects of EGCG for B[a]P-induced mutagenesis in vivo suggest that drinking green tea may reduce the tumor-initiating potency of B[a]P in the lung.

元の言語英語
ページ(範囲)421-424
ページ数4
ジャーナルCarcinogenesis
20
発行部数3
DOI
出版物ステータス出版済み - 1 1 1999

Fingerprint

Benzo(a)pyrene
Mutagenesis
Transgenic Mice
Lung
Mutation
Genes
Tea
Codon
Antimutagenic Agents
Injections
Guanine
Mutation Rate
epigallocatechin gallate
Base Pairing
Drinking Water
Drinking
Neoplasms
Water
Therapeutics

All Science Journal Classification (ASJC) codes

  • Cancer Research

これを引用

Inhibition of benzo[a]pyrene-induced mutagenesis by (-)-epigallocatechin gallate in the lung of rpsL transgenic mice. / Muto, Shigeharu; Yokoi, Tsuyoshi; Gondo, Yoichi; Katsuki, Motoya; Shioyama, Yoshiyuki; Fujita, Ken Ichi; Kamataki, Tetsuya.

:: Carcinogenesis, 巻 20, 番号 3, 01.01.1999, p. 421-424.

研究成果: ジャーナルへの寄稿記事

Muto, Shigeharu ; Yokoi, Tsuyoshi ; Gondo, Yoichi ; Katsuki, Motoya ; Shioyama, Yoshiyuki ; Fujita, Ken Ichi ; Kamataki, Tetsuya. / Inhibition of benzo[a]pyrene-induced mutagenesis by (-)-epigallocatechin gallate in the lung of rpsL transgenic mice. :: Carcinogenesis. 1999 ; 巻 20, 番号 3. pp. 421-424.
@article{57a9f81226cc4e38815531fcd1afbd9c,
title = "Inhibition of benzo[a]pyrene-induced mutagenesis by (-)-epigallocatechin gallate in the lung of rpsL transgenic mice",
abstract = "Epigallocatechin gallate (EGCG) is a major water-soluble component of green tea. The antimutagenic activity of EGCG against benzo[a]pyrene (B[a]P)-induced mutations was assessed by using transgenic mice carrying the rpsL gene as a monitor of mutations. Seven-week-old male mice were given drinking water containing EGCG for 3 weeks. On day 7, mice were treated with a single i.p. injection of B[a]P (500 mg/kg body wt). Two weeks after the injection, the mutations in the rpsL gene were analyzed. B[a]P treatment resulted in an ~ 4-fold increase of mutation frequency at the rpsL gene in the lung. An ~ 60{\%} reduction in the B[a]P-induced mutations in the lung was observed when mice were given EGCG at concentrations > 0.005{\%}. B[a]P-induced mutations mainly occurred at G:C base-pairs in the several specific nucleotide sequences of the rpsL gene. These were AGG, CGG, CGT, TGG, TGC and GGT: all of them contained a guanine residue. Mutations seen similarly in the human Ki-ras codon 12 or p53 codons 157, 248, and 273 of lung tumor were also found in the rpsL gene, and the mutations were suppressed by the EGCG treatment. In conclusion, the antimutagenic effects of EGCG for B[a]P-induced mutagenesis in vivo suggest that drinking green tea may reduce the tumor-initiating potency of B[a]P in the lung.",
author = "Shigeharu Muto and Tsuyoshi Yokoi and Yoichi Gondo and Motoya Katsuki and Yoshiyuki Shioyama and Fujita, {Ken Ichi} and Tetsuya Kamataki",
year = "1999",
month = "1",
day = "1",
doi = "10.1093/carcin/20.3.421",
language = "English",
volume = "20",
pages = "421--424",
journal = "Carcinogenesis",
issn = "0143-3334",
publisher = "Oxford University Press",
number = "3",

}

TY - JOUR

T1 - Inhibition of benzo[a]pyrene-induced mutagenesis by (-)-epigallocatechin gallate in the lung of rpsL transgenic mice

AU - Muto, Shigeharu

AU - Yokoi, Tsuyoshi

AU - Gondo, Yoichi

AU - Katsuki, Motoya

AU - Shioyama, Yoshiyuki

AU - Fujita, Ken Ichi

AU - Kamataki, Tetsuya

PY - 1999/1/1

Y1 - 1999/1/1

N2 - Epigallocatechin gallate (EGCG) is a major water-soluble component of green tea. The antimutagenic activity of EGCG against benzo[a]pyrene (B[a]P)-induced mutations was assessed by using transgenic mice carrying the rpsL gene as a monitor of mutations. Seven-week-old male mice were given drinking water containing EGCG for 3 weeks. On day 7, mice were treated with a single i.p. injection of B[a]P (500 mg/kg body wt). Two weeks after the injection, the mutations in the rpsL gene were analyzed. B[a]P treatment resulted in an ~ 4-fold increase of mutation frequency at the rpsL gene in the lung. An ~ 60% reduction in the B[a]P-induced mutations in the lung was observed when mice were given EGCG at concentrations > 0.005%. B[a]P-induced mutations mainly occurred at G:C base-pairs in the several specific nucleotide sequences of the rpsL gene. These were AGG, CGG, CGT, TGG, TGC and GGT: all of them contained a guanine residue. Mutations seen similarly in the human Ki-ras codon 12 or p53 codons 157, 248, and 273 of lung tumor were also found in the rpsL gene, and the mutations were suppressed by the EGCG treatment. In conclusion, the antimutagenic effects of EGCG for B[a]P-induced mutagenesis in vivo suggest that drinking green tea may reduce the tumor-initiating potency of B[a]P in the lung.

AB - Epigallocatechin gallate (EGCG) is a major water-soluble component of green tea. The antimutagenic activity of EGCG against benzo[a]pyrene (B[a]P)-induced mutations was assessed by using transgenic mice carrying the rpsL gene as a monitor of mutations. Seven-week-old male mice were given drinking water containing EGCG for 3 weeks. On day 7, mice were treated with a single i.p. injection of B[a]P (500 mg/kg body wt). Two weeks after the injection, the mutations in the rpsL gene were analyzed. B[a]P treatment resulted in an ~ 4-fold increase of mutation frequency at the rpsL gene in the lung. An ~ 60% reduction in the B[a]P-induced mutations in the lung was observed when mice were given EGCG at concentrations > 0.005%. B[a]P-induced mutations mainly occurred at G:C base-pairs in the several specific nucleotide sequences of the rpsL gene. These were AGG, CGG, CGT, TGG, TGC and GGT: all of them contained a guanine residue. Mutations seen similarly in the human Ki-ras codon 12 or p53 codons 157, 248, and 273 of lung tumor were also found in the rpsL gene, and the mutations were suppressed by the EGCG treatment. In conclusion, the antimutagenic effects of EGCG for B[a]P-induced mutagenesis in vivo suggest that drinking green tea may reduce the tumor-initiating potency of B[a]P in the lung.

UR - http://www.scopus.com/inward/record.url?scp=0033065391&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033065391&partnerID=8YFLogxK

U2 - 10.1093/carcin/20.3.421

DO - 10.1093/carcin/20.3.421

M3 - Article

C2 - 10190556

AN - SCOPUS:0033065391

VL - 20

SP - 421

EP - 424

JO - Carcinogenesis

JF - Carcinogenesis

SN - 0143-3334

IS - 3

ER -