Inhibition of DNA replication by an anti-PCNA aptamer/PCNA complex

Ewa Kowalska, Filip Bartnicki, Ryo Fujisawa, Piotr Bonarek, Pawet Hermanowicz, Toshiki Tsurimoto, Klaudia Muszynska, Wojciech Strzalka

研究成果: ジャーナルへの寄稿記事

4 引用 (Scopus)

抄録

Proliferating cell nuclear antigen (PCNA) is a multifunctional protein present in the nuclei of eukaryotic cells that plays an important role as a component of the DNA replication machinery, as well as DNA repair systems. PCNA was recently proposed as a potential non-oncogenic target for anti-cancer therapy. In this study, using the Systematic Evolution of Ligands by EXponential enrichment (SELEX) method, we developed a short DNA aptamer that binds human PCNA. In the presence of PCNA, the anti-PCNA aptamer inhibited the activity of human DNA polymerase and at nM concentrations. Moreover, PCNA protected the anti-PCNA aptamer against the exonucleolytic activity of these DNA polymerases. Investigation of the mechanism of anti-PCNA aptamer-dependent inhibition of DNA replication revealed that the aptamer did not block formation, but was a component of PCNA/DNA polymerase or complexes. Additionally, the anti-PCNA aptamer competed with the primer-template DNA for binding to the PCNA/DNA polymerase or complex. Based on the observations, a model of anti-PCNA aptamer/PCNA complex-dependent inhibition of DNA replication was proposed.

元の言語英語
ページ(範囲)25-41
ページ数17
ジャーナルNucleic acids research
46
発行部数1
DOI
出版物ステータス出版済み - 1 1 2018

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Proliferating Cell Nuclear Antigen
DNA Replication
DNA-Directed DNA Polymerase
SELEX Aptamer Technique
Nucleotide Aptamers
DNA Primers
Eukaryotic Cells
Human Activities
DNA Repair

All Science Journal Classification (ASJC) codes

  • Genetics

これを引用

Kowalska, E., Bartnicki, F., Fujisawa, R., Bonarek, P., Hermanowicz, P., Tsurimoto, T., ... Strzalka, W. (2018). Inhibition of DNA replication by an anti-PCNA aptamer/PCNA complex. Nucleic acids research, 46(1), 25-41. https://doi.org/10.1093/nar/gkx1184

Inhibition of DNA replication by an anti-PCNA aptamer/PCNA complex. / Kowalska, Ewa; Bartnicki, Filip; Fujisawa, Ryo; Bonarek, Piotr; Hermanowicz, Pawet; Tsurimoto, Toshiki; Muszynska, Klaudia; Strzalka, Wojciech.

:: Nucleic acids research, 巻 46, 番号 1, 01.01.2018, p. 25-41.

研究成果: ジャーナルへの寄稿記事

Kowalska, E, Bartnicki, F, Fujisawa, R, Bonarek, P, Hermanowicz, P, Tsurimoto, T, Muszynska, K & Strzalka, W 2018, 'Inhibition of DNA replication by an anti-PCNA aptamer/PCNA complex', Nucleic acids research, 巻. 46, 番号 1, pp. 25-41. https://doi.org/10.1093/nar/gkx1184
Kowalska E, Bartnicki F, Fujisawa R, Bonarek P, Hermanowicz P, Tsurimoto T その他. Inhibition of DNA replication by an anti-PCNA aptamer/PCNA complex. Nucleic acids research. 2018 1 1;46(1):25-41. https://doi.org/10.1093/nar/gkx1184
Kowalska, Ewa ; Bartnicki, Filip ; Fujisawa, Ryo ; Bonarek, Piotr ; Hermanowicz, Pawet ; Tsurimoto, Toshiki ; Muszynska, Klaudia ; Strzalka, Wojciech. / Inhibition of DNA replication by an anti-PCNA aptamer/PCNA complex. :: Nucleic acids research. 2018 ; 巻 46, 番号 1. pp. 25-41.
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