Inhibition of M-type K+ current by linopirdine, a neurotransmitter- release enhancer, in NG108-15 neuronal cells and rat cerebral neurons in culture

Mami Noda, Motoo Obana, Norio Akaike

研究成果: Contribution to journalArticle査読

18 被引用数 (Scopus)

抄録

The effect of linopirdine, a neurotransmitter-release enhancer, on the M-type K+-current, I(K(M)), was examined in NGPM1-27 cells, mouse neuroblastoma X rat glioma NG108-15 cells transformed to express m1- muscarinic acetylcholine (ACh) receptors, using the nystatin-perforated patch-recording mode under voltage-clamp conditions. The application of linopirdine induced the inward current associated with an inhibition of I(K(M)), which mimics an excitatory part of the ACh-induced responses in NGPM1-27 cells. The affinity of linopirdine for the inhibition of I(K(M)) was 24.7 μM in NGPM1-27 cells. In the presence of linopirdine, ACh failed to evoke a further inward current, but ACh still elicited an outward current, thus suggesting that the Ca2+-dependent K+ current is rather insensitive to linopirdine. Linopirdine also inhibited another voltage-gated potassium current (I(K(V)) at the concentration of 72.3 μM. Finally, the inhibitory effect of linopirdine on I(K(M)) was confirmed in pyramidal neurons acutely dissociated from the rat cerebral cortex at 35.8 μM. The results suggest that linopirdine is thus considered to be an inhibitor of some type of K+ channels in both NGPM1-27 cells and the rat cerebral neurons.

本文言語英語
ページ(範囲)274-280
ページ数7
ジャーナルBrain Research
794
2
DOI
出版ステータス出版済み - 6 1 1998

All Science Journal Classification (ASJC) codes

  • 神経科学(全般)
  • 分子生物学
  • 臨床神経学
  • 発生生物学

フィンガープリント

「Inhibition of M-type K<sup>+</sup> current by linopirdine, a neurotransmitter- release enhancer, in NG108-15 neuronal cells and rat cerebral neurons in culture」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル