Inhibition of Na+/H+ exchanger reduces infarct volume of focal cerebral ischemia in rats

Jiro Kitayama, Takanari Kitazono, Hiroshi Yao, Hiroaki Ooboshi, Hitonori Takaba, Tetsuro Ago, Masatoshi Fujishima, Setsuro Ibayashi

研究成果: ジャーナルへの寄稿記事

34 引用 (Scopus)

抄録

Activation of Na+/H+ exchanger (NHE) may have an important role in ischemic cell death by means of intracellular overload of Na+ and Ca2+. Recent evidence has suggested that inhibitors of NHE have protective effects on myocardial ischemia both in vivo and in vitro. In this study, we tested the hypothesis that FR183998, an inhibitor of NHE, reduces infarct volume produced by focal cerebral ischemia in rats. We used 20 male spontaneously hypertensive rats. Either FR183998 (1 mg/kg; n=10), or vehicle (n=10) was given intravenously to the rats and the distal middle cerebral artery of each animal was occluded using a photothrombotic technique. We measured regional cerebral blood flow using laser-Doppler flowmetry throughout the experiments. After 3 days, infarct volume was measured in each animal group. To estimate the brain edema, we also calculated the cortical volume in both hemispheres. The infarct volume in the FR183998-treated group (82±8 mm3, mean±S.E.M.) was significantly smaller than that in the control group (115±12 mm3) (P=0.034). The cortical volume of the occluded side in the FR183998-treated group (359±7 mm3) tended to be smaller than that in the control group (378±9 mm3) (P=0.116). The regional cerebral blood flow and physiological variables during ischemia were not significantly different between the two groups throughout the experiments. These results suggest that inhibition of NHE by FR183998 may have beneficial effects in reducing infarct volume and brain edema during cerebral ischemia. Thus, NHE may play an important role in the development of neuronal damage during acute cerebral ischemia.

元の言語英語
ページ(範囲)223-228
ページ数6
ジャーナルBrain Research
922
発行部数2
DOI
出版物ステータス出版済み - 12 20 2001

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Sodium-Hydrogen Antiporter
Brain Ischemia
Cerebrovascular Circulation
Brain Edema
Regional Blood Flow
Control Groups
Laser-Doppler Flowmetry
Middle Cerebral Artery
Inbred SHR Rats
Myocardial Ischemia
Cell Death
Ischemia

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

これを引用

Inhibition of Na+/H+ exchanger reduces infarct volume of focal cerebral ischemia in rats. / Kitayama, Jiro; Kitazono, Takanari; Yao, Hiroshi; Ooboshi, Hiroaki; Takaba, Hitonori; Ago, Tetsuro; Fujishima, Masatoshi; Ibayashi, Setsuro.

:: Brain Research, 巻 922, 番号 2, 20.12.2001, p. 223-228.

研究成果: ジャーナルへの寄稿記事

Kitayama, J, Kitazono, T, Yao, H, Ooboshi, H, Takaba, H, Ago, T, Fujishima, M & Ibayashi, S 2001, 'Inhibition of Na+/H+ exchanger reduces infarct volume of focal cerebral ischemia in rats', Brain Research, 巻. 922, 番号 2, pp. 223-228. https://doi.org/10.1016/S0006-8993(01)03175-4
Kitayama, Jiro ; Kitazono, Takanari ; Yao, Hiroshi ; Ooboshi, Hiroaki ; Takaba, Hitonori ; Ago, Tetsuro ; Fujishima, Masatoshi ; Ibayashi, Setsuro. / Inhibition of Na+/H+ exchanger reduces infarct volume of focal cerebral ischemia in rats. :: Brain Research. 2001 ; 巻 922, 番号 2. pp. 223-228.
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AU - Yao, Hiroshi

AU - Ooboshi, Hiroaki

AU - Takaba, Hitonori

AU - Ago, Tetsuro

AU - Fujishima, Masatoshi

AU - Ibayashi, Setsuro

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AB - Activation of Na+/H+ exchanger (NHE) may have an important role in ischemic cell death by means of intracellular overload of Na+ and Ca2+. Recent evidence has suggested that inhibitors of NHE have protective effects on myocardial ischemia both in vivo and in vitro. In this study, we tested the hypothesis that FR183998, an inhibitor of NHE, reduces infarct volume produced by focal cerebral ischemia in rats. We used 20 male spontaneously hypertensive rats. Either FR183998 (1 mg/kg; n=10), or vehicle (n=10) was given intravenously to the rats and the distal middle cerebral artery of each animal was occluded using a photothrombotic technique. We measured regional cerebral blood flow using laser-Doppler flowmetry throughout the experiments. After 3 days, infarct volume was measured in each animal group. To estimate the brain edema, we also calculated the cortical volume in both hemispheres. The infarct volume in the FR183998-treated group (82±8 mm3, mean±S.E.M.) was significantly smaller than that in the control group (115±12 mm3) (P=0.034). The cortical volume of the occluded side in the FR183998-treated group (359±7 mm3) tended to be smaller than that in the control group (378±9 mm3) (P=0.116). The regional cerebral blood flow and physiological variables during ischemia were not significantly different between the two groups throughout the experiments. These results suggest that inhibition of NHE by FR183998 may have beneficial effects in reducing infarct volume and brain edema during cerebral ischemia. Thus, NHE may play an important role in the development of neuronal damage during acute cerebral ischemia.

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