Inhibition of PTEN tumor suppressor promotes the generation of induced pluripotent stem cells

Jiyuan Liao, Tomotoshi Marumoto, Saori Yamaguchi, Shinji Okano, Naoki Takeda, Chika Sakamoto, Hirotaka Kawano, Takenobu Nii, Shohei Miyamato, Yoko Nagai, Michiyo Okada, Hiroyuki Inoue, Kohichi Kawahara, Akira Suzuki, Yoshie Miura, Kenzaburo Tani

研究成果: Contribution to journalArticle査読

29 被引用数 (Scopus)

抄録

Induced pluripotent stem cells (iPSCs) can be generated from patients with specific diseases by the transduction of reprogramming factors and can be useful as a cell source for cell transplantation therapy for various diseases with impaired organs. However, the low efficiency of iPSC derived from somatic cells (0.01-0.1%) is one of the major problems in the field. The phosphoinositide 3-kinase (PI3K) pathway is thought to be important for self-renewal, proliferation, and maintenance of embryonic stem cells (ESCs), but the contribution of this pathway or its well-known negative regulator, phosphatase, and tensin homolog deleted on chromosome ten (Pten), to somatic cell reprogramming remains largely unknown. Here, we show that activation of the PI3K pathway by the Pten inhibitor, dipotassium bisperoxo(5-hydroxypyridine-2- carboxyl)oxovanadate, improves the efficiency of germline-competent iPSC derivation from mouse somatic cells. This simple method provides a new approach for efficient generation of iPSCs.

本文言語英語
ページ(範囲)1242-1250
ページ数9
ジャーナルMolecular Therapy
21
6
DOI
出版ステータス出版済み - 6 2013

All Science Journal Classification (ASJC) codes

  • 分子医療
  • 分子生物学
  • 遺伝学
  • 薬理学
  • 創薬

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