TY - JOUR
T1 - Inhibition of S-Phase entry of human fibroblasts by an antisense oligomer against hCDC47
AU - Fujita, Masatoshi
AU - Kiyono, Tohru
AU - Hayashi, Yasuyuki
AU - Ishibashi, Masahide
N1 - Funding Information:
We thank Drs. R. Ueda and M. Seto (Laboratory of Chemotherapy, Research Institute, Aichi Cancer Center, Japan) for generously providing antibodies and T. Yoshida for technical assistance. This work was supported in part by a Grant-in-Aid for Cancer Research from the Ministry of Education, Science and Culture of Japan.
PY - 1996/2/15
Y1 - 1996/2/15
N2 - The MCM protein family has been implicated in the regulatory machinery causing DNA to replicate only once in the S phase. Recently, we have identified human (h) CDC47, another member of the family, and demonstrated its unique S-phase nuclear association pattern implying participation in the regulation of DNA replication. In the present study, we applied an antisense strategy to assess the phenotype of human cells in which hCDC47 is repressed. As a result serum-induced accumulation of hCDC47 protein in human fibroblast cells was reduced by a specific antisense oligodeoxynucleotide and S-phase entry of such cells was significantly inhibited, indicating that hCDC47 protein is essential for DNA replication.
AB - The MCM protein family has been implicated in the regulatory machinery causing DNA to replicate only once in the S phase. Recently, we have identified human (h) CDC47, another member of the family, and demonstrated its unique S-phase nuclear association pattern implying participation in the regulation of DNA replication. In the present study, we applied an antisense strategy to assess the phenotype of human cells in which hCDC47 is repressed. As a result serum-induced accumulation of hCDC47 protein in human fibroblast cells was reduced by a specific antisense oligodeoxynucleotide and S-phase entry of such cells was significantly inhibited, indicating that hCDC47 protein is essential for DNA replication.
UR - http://www.scopus.com/inward/record.url?scp=0029670350&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029670350&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1996.0280
DO - 10.1006/bbrc.1996.0280
M3 - Article
C2 - 8605034
AN - SCOPUS:0029670350
SN - 0006-291X
VL - 219
SP - 604
EP - 607
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -