Inhibition of Starfish Oocyte Maturation by Tumor‐Promoting Phorbol Esters: starfish oocyte maturation/tumor promoters/phorbol esters/teleocidin/retinoids

TAKEO KISHIMOTO, Michiyasu Yoshikuni, HIROYUKI IKADAI, HARUO KANATANI

研究成果: Contribution to journalArticle査読

11 被引用数 (Scopus)

抄録

Effect of tumor promoters including phorbol esters and teleocidin on 1‐methyladenine (1‐MeAde)‐induced oocyte maturation was studied in the starfish. When isolated immature oocytes were treated with 1‐MeAde and 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA), 1‐MeAde‐induced maturation was completely inhibited at more than 2.5 μg/ml. However, if TPA was added after the hormone‐dependent period (the minimum period wherein 1‐MeAde is required), such maturation‐inhibiting effect was no longer observed. Pretreatment with TPA for 5 min showed that its inhibitory action is irreversible. However, when TPA‐injected oocytes were treated with 1‐MeAde, all oocytes underwent germinal vesicle breakdown (GVBD). GVBD was induced in TPA‐treated oocytes upon injection of the cytoplasm of maturing oocytes containing maturation‐promoting factor (MPF). These facts show that TPA acts on the oocyte surface to inhibit the production of MPF. Retinoids including retinal, retinol and retinoic acid reversed the inhibitory effect of TPA on 1‐MeAde‐induced maturation. Experiments with various phorbol esters showed a good correlation between their maturation‐inhibiting activity and their known tumor‐promoting activity. Further, telecoidin, which is structurally unrelated to phorbol esters, inhibited 1‐MeAde action. Since both tumor‐promoting phorbol esters and teleocidin are known to activate Ca2+ ‐activated, phospholipid‐dependent protein kinase (protein kinase C) and their activation effect is inhibited by retinoids, it appears that the activation of protein kinase C by tumor promoters is involved in blocking of 1‐MeAde action.

本文言語英語
ページ(範囲)233-242
ページ数10
ジャーナルDevelopment, Growth & Differentiation
27
3
DOI
出版ステータス出版済み - 1 1 1985
外部発表はい

All Science Journal Classification (ASJC) codes

  • 発生生物学
  • 細胞生物学

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