Inhibitory Effect on Thrombin-induced Myosin Light Chain Phosphorylation by Extract of a New Green Tea Cultivar in A7r5 Rat Aortic Smooth Muscle Cells

Kana Kurihara, Shunsuke Nagatomo, Mari Maeda-Yamamoto, Koji Yamada, Hirofumi Tachibana

研究成果: ジャーナルへの寄稿記事

1 引用 (Scopus)

抄録

We show that an extract of a new green tea cultivar Cha Chuukanbohon Nou6 (Nou6), which rich in anthocyanin, has an inhibitory effect on myosin regulatory light chain (MRLC) phosphorylation in rat aortic smooth muscle cell line A7r5. The inhibitory effect of Nou6 extract was abolished when the extract was treated with polyvinylpyrrolidone which traps polyphenols. However, none of the major tea constituents tested had an inhibitory effect. In addition, we found that Nou6 extract had a strong effect on reducing the phosphorylation of myosin phosphatase targeting subunit 1 at Thr696. These results suggest that Nou6 extract inhibits MRLC phosphorylation induced by thrombin through activating myosin light chain phosphatase, and polyphenols in the tea extract may contribute to this effect.

元の言語英語
ページ(範囲)263-266
ページ数4
ジャーナルFood Science and Technology Research
16
発行部数3
DOI
出版物ステータス出版済み - 5 2010

Fingerprint

myosin light chains
Myosin Light Chains
Phosphorylation
thrombin
green tea
Tea
Myosin-Light-Chain Phosphatase
Thrombin
smooth muscle
myocytes
Smooth Muscle Myocytes
Muscle
Rats
phosphorylation
Phosphatases
Cells
Polyphenols
rats
extracts
cultivars

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering
  • Marketing

これを引用

Inhibitory Effect on Thrombin-induced Myosin Light Chain Phosphorylation by Extract of a New Green Tea Cultivar in A7r5 Rat Aortic Smooth Muscle Cells. / Kurihara, Kana; Nagatomo, Shunsuke; Maeda-Yamamoto, Mari; Yamada, Koji; Tachibana, Hirofumi.

:: Food Science and Technology Research, 巻 16, 番号 3, 05.2010, p. 263-266.

研究成果: ジャーナルへの寄稿記事

@article{03e497af14664694b16951aa02ff562e,
title = "Inhibitory Effect on Thrombin-induced Myosin Light Chain Phosphorylation by Extract of a New Green Tea Cultivar in A7r5 Rat Aortic Smooth Muscle Cells",
abstract = "We show that an extract of a new green tea cultivar Cha Chuukanbohon Nou6 (Nou6), which rich in anthocyanin, has an inhibitory effect on myosin regulatory light chain (MRLC) phosphorylation in rat aortic smooth muscle cell line A7r5. The inhibitory effect of Nou6 extract was abolished when the extract was treated with polyvinylpyrrolidone which traps polyphenols. However, none of the major tea constituents tested had an inhibitory effect. In addition, we found that Nou6 extract had a strong effect on reducing the phosphorylation of myosin phosphatase targeting subunit 1 at Thr696. These results suggest that Nou6 extract inhibits MRLC phosphorylation induced by thrombin through activating myosin light chain phosphatase, and polyphenols in the tea extract may contribute to this effect.",
author = "Kana Kurihara and Shunsuke Nagatomo and Mari Maeda-Yamamoto and Koji Yamada and Hirofumi Tachibana",
year = "2010",
month = "5",
doi = "10.3136/fstr.16.263",
language = "English",
volume = "16",
pages = "263--266",
journal = "Food Science and Technology Research",
issn = "1344-6606",
publisher = "Japanese Society for Food Science and Technology",
number = "3",

}

TY - JOUR

T1 - Inhibitory Effect on Thrombin-induced Myosin Light Chain Phosphorylation by Extract of a New Green Tea Cultivar in A7r5 Rat Aortic Smooth Muscle Cells

AU - Kurihara, Kana

AU - Nagatomo, Shunsuke

AU - Maeda-Yamamoto, Mari

AU - Yamada, Koji

AU - Tachibana, Hirofumi

PY - 2010/5

Y1 - 2010/5

N2 - We show that an extract of a new green tea cultivar Cha Chuukanbohon Nou6 (Nou6), which rich in anthocyanin, has an inhibitory effect on myosin regulatory light chain (MRLC) phosphorylation in rat aortic smooth muscle cell line A7r5. The inhibitory effect of Nou6 extract was abolished when the extract was treated with polyvinylpyrrolidone which traps polyphenols. However, none of the major tea constituents tested had an inhibitory effect. In addition, we found that Nou6 extract had a strong effect on reducing the phosphorylation of myosin phosphatase targeting subunit 1 at Thr696. These results suggest that Nou6 extract inhibits MRLC phosphorylation induced by thrombin through activating myosin light chain phosphatase, and polyphenols in the tea extract may contribute to this effect.

AB - We show that an extract of a new green tea cultivar Cha Chuukanbohon Nou6 (Nou6), which rich in anthocyanin, has an inhibitory effect on myosin regulatory light chain (MRLC) phosphorylation in rat aortic smooth muscle cell line A7r5. The inhibitory effect of Nou6 extract was abolished when the extract was treated with polyvinylpyrrolidone which traps polyphenols. However, none of the major tea constituents tested had an inhibitory effect. In addition, we found that Nou6 extract had a strong effect on reducing the phosphorylation of myosin phosphatase targeting subunit 1 at Thr696. These results suggest that Nou6 extract inhibits MRLC phosphorylation induced by thrombin through activating myosin light chain phosphatase, and polyphenols in the tea extract may contribute to this effect.

UR - http://www.scopus.com/inward/record.url?scp=77954263515&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77954263515&partnerID=8YFLogxK

U2 - 10.3136/fstr.16.263

DO - 10.3136/fstr.16.263

M3 - Article

AN - SCOPUS:77954263515

VL - 16

SP - 263

EP - 266

JO - Food Science and Technology Research

JF - Food Science and Technology Research

SN - 1344-6606

IS - 3

ER -