Inhibitory effects of the leaves of loquat (eriobotrya japonica) on bone mineral density loss in ovariectomized mice and osteoclast differentiation

Hui Tan, Syoko Furuta, Toshiro Nagata, Koichiro Ohnuki, Taiki Akasaka, Bungo Shirouchi, Masao Sato, Ryuichiro Kondo, Kuniyoshi Shimizu

研究成果: Contribution to journalArticle査読

21 被引用数 (Scopus)

抄録

The loquat, Eriobotrya japonica Lindl. (Rosaceae), is a small tree native to Japan and China that is widely cultivated for its succulent fruit. Its leaves are used as an ingredient of a tasty tea called "Biwa cha" in Japanese. The anti-osteoporosis effects of the leaves of loquat in vitro and in vivo have been investigated. After 15 days of feeding normal diet or diet supplemented with 5% loquat leaves, the body weight, viscera weights, and bone mineral density (BMD) of both groups of eight ovariectomized (OVX) mice were compared. The result showed that the loss of BMD in loquat-fed mice was significantly prevented in three parts of the body, especially in the trabecular bone of the head (P < 0.05), abdomen (P < 0.01), and lumbar (P < 0.05) compared to the control group. No hypertrophy in the uterus by the loquat leaves diet was observed. The effect of the extract (447.25 g) prepared from the dried leaves of loquat (2.36 kg) was further studied on RANKL-induced osteoclast differentiation and cell viability. The extract suppressed the differentiation of osteoclasts under 50, 125, 250, and 500 μg/mL. Through bioactivity-guided fractionation, ursolic acid (1) was isolated and inhibited osteoclast differentiation under 4 and 10 μg/mL. It was concluded that loquat leaves possess the potential to suppress ovariectomy-induced bone mineral density deterioration.

本文言語英語
ページ(範囲)836-841
ページ数6
ジャーナルJournal of Agricultural and Food Chemistry
62
4
DOI
出版ステータス出版済み - 1 29 2014

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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