Initial step of B 12-dependent enzymatic catalysis: Energetic implications regarding involvement of the one-electron-reduced form of adenosylcobalamin cofactor

Pawel M. Kozlowski, Takashi Kamachi, Manoj Kumar, Kazunari Yoshizawa

研究成果: ジャーナルへの寄稿学術誌査読

9 被引用数 (Scopus)

抄録

Density functional theory analysis was performed to elucidate the impact of one-electron reduction upon the initial step of adenosylcobalamin-dependent enzymatic catalysis. The transition state (TS) corresponding to the Co-C bond cleavage and subsequent hydrogen abstraction from the substrate was located. The intrinsic reaction coordinate calculations predicted that the reaction consisting of Co-C5' bond cleavage in [Co III(corrin )]-Rib (where Rib is ribosyl) and hydrogen-atom abstraction from the CH 3-CH 2-CHO substrate occurs in a concerted fashion. The computed activation energy barrier of the reaction (15.0 kcal/mol) was lowered by approximately 54.5% in comparison with the reaction involving the positively charged cofactor model (Im-[Co III(corrin)]-Rib +, where Im is imidazole; energy barrier = 33.0 kcal/mol). The Im base was detached during the TS search in the reaction involving the one-electron-reduced analogue. Thus, to compare the energetics of the two reactions, the axial Im ligand detachment energy for the Im-[Co III(corrin )]-Rib model was computed [7.6 kcal/mol (gas phase); 4.6 kcal/mol (water)]. Consequently, the effective activation energy barrier for the reaction mediated by the Im-off [Co III (corrin )]-Rib was estimated to be 22.6 kcal/mol, which implied an overall 31.5% reduction in the energetic demands of the reaction. Considering that the lengthened Co-N axial bond has been observed in X-ray crystal structure studies of B 12-dependent mutases, the catalytic impact induced by one-electron reduction of the cofactor is expected to be higher in the presence of the enzymatic environment.

本文言語英語
ページ(範囲)293-300
ページ数8
ジャーナルJournal of Biological Inorganic Chemistry
17
2
DOI
出版ステータス出版済み - 2月 2012

!!!All Science Journal Classification (ASJC) codes

  • 生化学
  • 無機化学

フィンガープリント

「Initial step of B 12-dependent enzymatic catalysis: Energetic implications regarding involvement of the one-electron-reduced form of adenosylcobalamin cofactor」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル