Inositol 1,4,5-trisphosphate affinity chromatography

Masato Hirata, Yutaka Watanabe, Toyohiro Ishimatsu, Fumi Yanaga, Toshitaka Koga, Shoichiro Ozaki

研究成果: Contribution to journalArticle査読

50 被引用数 (Scopus)

抄録

Inositol 1,4,5-trisphosphate (IP3) affinity columns were made by coupling IP3 analogs to a supporting matrix, Sepharose 4B. IP3 5-phosphatase activity, IP3 3-kinase activity and IP3 binding activity from rat brain were adsorbed on the IP3 columns, and were eluted by increasing KCl concentration. This purification procedure increased the specific activities of these parameters 5-200-fold. Thus Sepharose 4B immobilized IP3 analogs can specifically interact with IP3-binding proteins, demonstrating that IP3 affinity columns are a good method for purifying such proteins. Furthermore, our results suggest that IP3 analogs can be linked to other molecules to make useful derivatives without loss of their biological activities.

本文言語英語
ページ(範囲)379-386
ページ数8
ジャーナルBiochemical and Biophysical Research Communications
168
1
DOI
出版ステータス出版済み - 4 16 1990

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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