Inositol 1,4,5-trisphosphate and intracellular Ca 2+ store sites in human peripheral lymphocytes

E. Suematsu, J. Nishimura, Masato Hirata

研究成果: ジャーナルへの寄稿記事

3 引用 (Scopus)

抄録

Ca 2+ storage by vesicles in human lymphocytes was studied using saponin-permeabilized cells. Saponin-treated lymphocytes could accumulate Ca 2+ in the presence of ATP. In the presence of NaN 3 (10 mM), the maximal storages of Ca 2+ (0.87 nmol/4 x 10 6 cells) was obtained at free Ca 2+ concentration of 10 -5 M, and the apparent affinity constant for Ca 2+ was approximately 1.4 x 10 6 M -1 . Stored Ca 2+ was rapidly released by A23187. Ca 2+ accumulation was markedly enhanced by oxalate, and precipates of Ca-oxalate were electron-microscopically observed inside the endoplasmic reticulum-like structures. In the absence of NaN 3 , the maximal amount of intracellular Ca 2+ was about 30 nmol/4 x 10 6 cells, while the affinity for Ca 2+ was much lower. Application of inositol 1,4,5-trisphosphate to saponin-permeabilized cells which had accumulated Ca 2+ by ATP in the presence of NaN 3 rapidly released 35% of stored Ca 2+ within 1 min. However, when the concentration of free Ca 2+ was higher than 1.5 x 10 -6 M, Ca 2+ release by this agent was inhibited. The results suggest that inositol 1,4,5-trisphosphate may function as a mediator in lymphocytes, linking activation of surface membrane receptors with mobilization of internal Ca 2+ , which plays an important role in lymphocyte responses involved in proliferation.

元の言語英語
ページ(範囲)279-286
ページ数8
ジャーナルBiomedical Research
6
発行部数5
出版物ステータス出版済み - 1 1 1985

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Inositol 1,4,5-Trisphosphate
Lymphocytes
Saponins
Oxalates
Adenosine Triphosphate
Calcimycin
Lymphocyte Activation
Endoplasmic Reticulum
Chemical activation
Electrons
Membranes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

これを引用

Inositol 1,4,5-trisphosphate and intracellular Ca 2+ store sites in human peripheral lymphocytes . / Suematsu, E.; Nishimura, J.; Hirata, Masato.

:: Biomedical Research, 巻 6, 番号 5, 01.01.1985, p. 279-286.

研究成果: ジャーナルへの寄稿記事

Suematsu, E, Nishimura, J & Hirata, M 1985, ' Inositol 1,4,5-trisphosphate and intracellular Ca 2+ store sites in human peripheral lymphocytes ', Biomedical Research, 巻. 6, 番号 5, pp. 279-286.
Suematsu, E. ; Nishimura, J. ; Hirata, Masato. / Inositol 1,4,5-trisphosphate and intracellular Ca 2+ store sites in human peripheral lymphocytes :: Biomedical Research. 1985 ; 巻 6, 番号 5. pp. 279-286.
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