Inositol trisphosphate/Ca2+ as messengers of bradykinin B2 and muscarinic acetylcholine m1-m4 receptors in neuroblastoma-derived hybrid cells

Mami Noda, Nobuto Ishizaka, Shigeru Yokoyama, Naoto Hoshi, Yasuhiro Kimura, Minako Hashii, Megumi Taketo, Alla Egorova, Rimma Knijnik, Kazuhiko Fukuda, Hitoshi Morikawa, David A. Brown, Haruhiro Higashida

研究成果: Contribution to journalArticle査読

15 被引用数 (Scopus)


Neuroblastoma x glioma hybrid NG108-15 and neuroblastoma x fibroblast hybrid NL308 cells possess endogenous bradykinin B2 receptors and m4 muscarinic acetylcholine receptors (mAChRs), which couple to phospholipase C and adenylate cyclase, respectively. Four genetic subtypes of mAChRs differed in their effects when stimulated in NG108-15 and NL308 cells overexpressing mAChRs. Broadly speaking, the principal effects fell into two categories: the odd-numbered receptors (m1 and m3) activated phospholipase C and increased inositol trisphosphate/Ca2+, as bradykinin did, whereas the even-numbered receptors (m2 and m4) inhibited adenylate cyclase via a pertussis toxin (PTx)-sensitive G-protein in NG108-15 cells. But all four types of NL308 cells overexpressing each m1, m2, m3 and m4 receptor activated phospholipase C, while keeping the PTx-sensitivity in m2/m4, but not in m1/m3 receptors. Coupling to ion channel effectors showed a comparable dichotomy in NG108-15 cells, while cross-activation occurred in NL308 cells.

ジャーナルJournal of Lipid Mediators and Cell Signalling
出版ステータス出版済み - 9 1996

All Science Journal Classification (ASJC) codes

  • Immunology
  • Pharmacology

フィンガープリント 「Inositol trisphosphate/Ca<sup>2+</sup> as messengers of bradykinin B<sub>2</sub> and muscarinic acetylcholine m1-m4 receptors in neuroblastoma-derived hybrid cells」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。