Insecticidal activity of the metalloprotease AprA occurs through suppression of host cellular and humoral immunity

Seung Ah Lee, Seong Han Jang, Byung Hyun Kim, Toshio Shibata, Jinwook Yoo, Yunjin Jung, Shun-Ichiro Kawabata, Bok Luel Lee

研究成果: ジャーナルへの寄稿記事

5 引用 (Scopus)

抄録

The biochemical characterization of virulence factors from entomopathogenic bacteria is important to understand entomopathogen-insect molecular interactions. Pseudomonas entomophila is a typical entomopathogenic bacterium that harbors virulence factors against several insects. However, the molecular actions of these factors against host innate immune responses are not clearly elucidated. In this study, we observed that bean bugs (Riptortus pedestris) that were injected with P. entomophila were highly susceptible to this bacterium. To determine how P. entomophila counteracts the host innate immunity to survive within the insect, we purified a highly enriched protein with potential host insect-killing activity from the culture supernatant of P. entomophila. Then, a 45-kDa protein was purified to homogeneity and identified as AprA which is an alkaline zinc metalloprotease of the genus Pseudomonas by liquid chromatography mass spectrometry (LC-MS). Purified AprA showed a pronounced killing effect against host insects and suppressed both host cellular and humoral innate immunity. Furthermore, to show that AprA is an important insecticidal protein of P. entomophila, we used an aprA-deficient P. entomophila mutant strain (ΔaprA). When ΔaprA mutant cells were injected to host insects, this mutant exhibited extremely attenuated virulence. In addition, the cytotoxicity against host hemocytes and the antimicrobial peptide-degrading ability of the ΔaprA mutant were greatly decreased. These findings suggest that AprA functions as an important insecticidal protein of P. entomophila via suppression of host cellular and humoral innate immune responses.

元の言語英語
ページ(範囲)116-126
ページ数11
ジャーナルDevelopmental and Comparative Immunology
81
DOI
出版物ステータス出版済み - 4 1 2018

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Metalloproteases
Humoral Immunity
Cellular Immunity
Insects
Innate Immunity
Virulence Factors
Pseudomonas
Bacteria
Proteins
Hemocytes
Liquid Chromatography
Virulence
Zinc
Mass Spectrometry
Peptides

All Science Journal Classification (ASJC) codes

  • Immunology
  • Developmental Biology

これを引用

Insecticidal activity of the metalloprotease AprA occurs through suppression of host cellular and humoral immunity. / Lee, Seung Ah; Jang, Seong Han; Kim, Byung Hyun; Shibata, Toshio; Yoo, Jinwook; Jung, Yunjin; Kawabata, Shun-Ichiro; Lee, Bok Luel.

:: Developmental and Comparative Immunology, 巻 81, 01.04.2018, p. 116-126.

研究成果: ジャーナルへの寄稿記事

Lee, Seung Ah ; Jang, Seong Han ; Kim, Byung Hyun ; Shibata, Toshio ; Yoo, Jinwook ; Jung, Yunjin ; Kawabata, Shun-Ichiro ; Lee, Bok Luel. / Insecticidal activity of the metalloprotease AprA occurs through suppression of host cellular and humoral immunity. :: Developmental and Comparative Immunology. 2018 ; 巻 81. pp. 116-126.
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abstract = "The biochemical characterization of virulence factors from entomopathogenic bacteria is important to understand entomopathogen-insect molecular interactions. Pseudomonas entomophila is a typical entomopathogenic bacterium that harbors virulence factors against several insects. However, the molecular actions of these factors against host innate immune responses are not clearly elucidated. In this study, we observed that bean bugs (Riptortus pedestris) that were injected with P. entomophila were highly susceptible to this bacterium. To determine how P. entomophila counteracts the host innate immunity to survive within the insect, we purified a highly enriched protein with potential host insect-killing activity from the culture supernatant of P. entomophila. Then, a 45-kDa protein was purified to homogeneity and identified as AprA which is an alkaline zinc metalloprotease of the genus Pseudomonas by liquid chromatography mass spectrometry (LC-MS). Purified AprA showed a pronounced killing effect against host insects and suppressed both host cellular and humoral innate immunity. Furthermore, to show that AprA is an important insecticidal protein of P. entomophila, we used an aprA-deficient P. entomophila mutant strain (ΔaprA). When ΔaprA mutant cells were injected to host insects, this mutant exhibited extremely attenuated virulence. In addition, the cytotoxicity against host hemocytes and the antimicrobial peptide-degrading ability of the ΔaprA mutant were greatly decreased. These findings suggest that AprA functions as an important insecticidal protein of P. entomophila via suppression of host cellular and humoral innate immune responses.",
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