Insecticidal bacterium isolated from an ant lion larva from Munakata, Japan

I. Egami, K. Iiyama, P. Zhang, Y. Chieda, N. Ino, K. Hasegawa, J. M. Lee, T. Kusakabe, C. Yasunaga-Aoki, S. Shimizu

研究成果: ジャーナルへの寄稿記事

11 引用 (Scopus)

抄録

Twenty bacteria were isolated from four ant lion larvae. The isolates were classified into three groups by biological characteristics. Since Group I, Group II and Group III were isolated from individual larvae Kuo1, Kuo3, 4 and Kuo2, respectively, with exception of one isolate Kuo2-1, each ant lion tested had its own dominant bacterial flora. Groups I and II were closer to Serratia liquefaciens and Enterobacter cloacae, respectively, whereas Group III could not be identified by the test used. The phylogenetic analysis of GroEL amino acid sequences revealed that Group I, II and III were related to those of Serratia spp., E. cloacae and Salmonella spp. -Escherichia/Shigella spp., respectively. Among these groups, Group I was highly virulent against Bombyx mori and Periplaneta americana, and caused 100% mortality within 24 h. The other two groups (Group II and III) were avirulent to these insect species. The culture filtrate of Group I caused killing activity to B. mori larvae and the insecticidal substance was purified from culture filtrate of Group I bacterium. Since the insecticidal activity highly correlated with proteolytic activity in the chromatographies, Group I bacterium may secret insecticidal proteinase in vitro.

元の言語英語
ページ(範囲)117-124
ページ数8
ジャーナルJournal of Applied Entomology
133
発行部数2
DOI
出版物ステータス出版済み - 3 1 2009

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Myrmeleontidae
Enterobacter cloacae
Serratia
culture filtrates
Japan
Bombyx mori
larvae
bacteria
Escherichia
Shigella
Periplaneta americana
Salmonella
chromatography
proteinases
amino acid sequences
flora
insects
phylogeny
testing

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Insect Science

これを引用

Insecticidal bacterium isolated from an ant lion larva from Munakata, Japan. / Egami, I.; Iiyama, K.; Zhang, P.; Chieda, Y.; Ino, N.; Hasegawa, K.; Lee, J. M.; Kusakabe, T.; Yasunaga-Aoki, C.; Shimizu, S.

:: Journal of Applied Entomology, 巻 133, 番号 2, 01.03.2009, p. 117-124.

研究成果: ジャーナルへの寄稿記事

Egami, I. ; Iiyama, K. ; Zhang, P. ; Chieda, Y. ; Ino, N. ; Hasegawa, K. ; Lee, J. M. ; Kusakabe, T. ; Yasunaga-Aoki, C. ; Shimizu, S. / Insecticidal bacterium isolated from an ant lion larva from Munakata, Japan. :: Journal of Applied Entomology. 2009 ; 巻 133, 番号 2. pp. 117-124.
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abstract = "Twenty bacteria were isolated from four ant lion larvae. The isolates were classified into three groups by biological characteristics. Since Group I, Group II and Group III were isolated from individual larvae Kuo1, Kuo3, 4 and Kuo2, respectively, with exception of one isolate Kuo2-1, each ant lion tested had its own dominant bacterial flora. Groups I and II were closer to Serratia liquefaciens and Enterobacter cloacae, respectively, whereas Group III could not be identified by the test used. The phylogenetic analysis of GroEL amino acid sequences revealed that Group I, II and III were related to those of Serratia spp., E. cloacae and Salmonella spp. -Escherichia/Shigella spp., respectively. Among these groups, Group I was highly virulent against Bombyx mori and Periplaneta americana, and caused 100{\%} mortality within 24 h. The other two groups (Group II and III) were avirulent to these insect species. The culture filtrate of Group I caused killing activity to B. mori larvae and the insecticidal substance was purified from culture filtrate of Group I bacterium. Since the insecticidal activity highly correlated with proteolytic activity in the chromatographies, Group I bacterium may secret insecticidal proteinase in vitro.",
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