Insertion and deletion mismatches distant from the target position improve gene correction with a tailed duplex

Hiroyuki Kamiya, Natsuki Nishigaki, Akihiro Ikeda, Seiya Yukawa, Yukiko Morita, Yoshimichi Nakatsu, Teruhisa Tsuzuki, Hideyoshi Harashima

研究成果: Contribution to journalArticle査読

3 被引用数 (Scopus)

抄録

A 5′-tailed duplex (TD) DNA corrects a base-substitution mutation. In this study, the effects of insertion and deletion (indel) mismatches distant from the target position on the gene correction were examined. Three target plasmid DNAs with and without indel mismatches ∼330 bases distant from the correction target position were prepared, and introduced into HeLa cells together with the TD. The indel mismatches improved the gene correction efficiency and specificity without sequence conversions at the indel mismatch site. These results suggested that the gene correction efficiency and specificity are increased when an appropriate second mismatch is introduced into the TD fragment.

本文言語英語
ページ(範囲)379-388
ページ数10
ジャーナルNucleosides, Nucleotides and Nucleic Acids
35
7
DOI
出版ステータス出版済み - 7 2 2016

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Genetics

フィンガープリント 「Insertion and deletion mismatches distant from the target position improve gene correction with a tailed duplex」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル