Insight into functional diversity of cytochrome P450 in the white-rot basidiomycete Phanerochaete chrysosporium: Involvement of versatile monooxygenase

Shinji Hirosue, Masahiro Tazaki, Nobuhiro Hiratsuka, Satoshi Yanai, Hiroki Kabumoto, Raku Shinkyo, Akira Arisawa, Toshiyuki Sakaki, Hiroshi Tsunekawa, Osamu Johdo, Hirofumi Ichinose, Hiroyuki Wariishi

研究成果: ジャーナルへの寄稿記事

41 引用 (Scopus)

抄録

To elucidate functional diversity of cytochrome P450 monooxygenases from the white-rot basidiomycete Phanerochaete chrysosporium (PcCYPs), we conducted a comprehensive functional screening using a wide variety of compounds. A functionomic survey resulted in characterization of novel PcCYP functions and discovery of versatile PcCYPs that exhibit broad substrate profiles. These results suggested that multifunctional properties of the versatile PcCYPs would play crucial roles in diversification of fungal metabolic systems involved in xenobiotic detoxification. To our knowledge, this is the first report describing multifunctional properties of versatile P450s from the fungal kingdom. An increased compilation of PcCYP functions will facilitate a thorough understanding of metabolic diversity in basidiomycetes and provide new insights that could also expedite practical applications in the biotechnology sector.

元の言語英語
ページ(範囲)118-123
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
407
発行部数1
DOI
出版物ステータス出版済み - 4 1 2011

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Phanerochaete
Basidiomycota
Mixed Function Oxygenases
Cytochrome P-450 Enzyme System
Detoxification
Xenobiotics
Biotechnology
Screening
Substrates

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Insight into functional diversity of cytochrome P450 in the white-rot basidiomycete Phanerochaete chrysosporium : Involvement of versatile monooxygenase. / Hirosue, Shinji; Tazaki, Masahiro; Hiratsuka, Nobuhiro; Yanai, Satoshi; Kabumoto, Hiroki; Shinkyo, Raku; Arisawa, Akira; Sakaki, Toshiyuki; Tsunekawa, Hiroshi; Johdo, Osamu; Ichinose, Hirofumi; Wariishi, Hiroyuki.

:: Biochemical and Biophysical Research Communications, 巻 407, 番号 1, 01.04.2011, p. 118-123.

研究成果: ジャーナルへの寄稿記事

Hirosue, Shinji ; Tazaki, Masahiro ; Hiratsuka, Nobuhiro ; Yanai, Satoshi ; Kabumoto, Hiroki ; Shinkyo, Raku ; Arisawa, Akira ; Sakaki, Toshiyuki ; Tsunekawa, Hiroshi ; Johdo, Osamu ; Ichinose, Hirofumi ; Wariishi, Hiroyuki. / Insight into functional diversity of cytochrome P450 in the white-rot basidiomycete Phanerochaete chrysosporium : Involvement of versatile monooxygenase. :: Biochemical and Biophysical Research Communications. 2011 ; 巻 407, 番号 1. pp. 118-123.
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