Inter-laboratory comparison of metabolite measurements for metabolomics data integration

Yoshihiro Izumi, Fumio Matsuda, Akiyoshi Hirayama, Kazutaka Ikeda, Yoshihiro Kita, Kanta Horie, Daisuke Saigusa, Kosuke Saito, Yuji Sawada, Hiroki Nakanishi, Nobuyuki Okahashi, Masatomo Takahashi, Motonao Nakao, Kosuke Hata, Yutaro Hoshi, Motohiko Morihara, Kazuhiro Tanabe, Takeshi Bamba, Yoshiya Oda

研究成果: ジャーナルへの寄稿記事

抄録

Background: One of the current problems in the field of metabolomics is the difficulty in integrating data collected using different equipment at different facilities, because many metabolomic methods have been developed independently and are unique to each laboratory. Methods: In this study, we examined whether different analytical methods among 12 different laboratories provided comparable relative quantification data for certain metabolites. Identical samples extracted from two cell lines (HT-29 and AsPc-1) were distributed to each facility, and hydrophilic and hydrophobic metabolite analyses were performed using the daily routine protocols of each laboratory. Results: The results indicate that there was no difference in the relative quantitative data (HT-29/AsPc-1) for about half of the measured metabolites among the laboratories and assay methods. Data review also revealed that errors in relative quantification were derived from issues such as erroneous peak identification, insufficient peak separation, a difference in detection sensitivity, derivatization reactions, and extraction solvent interference. Conclusion: The results indicated that relative quantification data obtained at different facilities and at different times would be integrated and compared by using a reference materials shared for data normalization.

元の言語英語
記事番号257
ジャーナルMetabolites
9
発行部数11
DOI
出版物ステータス出版済み - 11 2019

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Metabolomics
Data integration
Metabolites
Solvent extraction
Assays
Cells
Cell Line
Equipment and Supplies

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology

これを引用

Izumi, Y., Matsuda, F., Hirayama, A., Ikeda, K., Kita, Y., Horie, K., ... Oda, Y. (2019). Inter-laboratory comparison of metabolite measurements for metabolomics data integration. Metabolites, 9(11), [257]. https://doi.org/10.3390/metabo9110257

Inter-laboratory comparison of metabolite measurements for metabolomics data integration. / Izumi, Yoshihiro; Matsuda, Fumio; Hirayama, Akiyoshi; Ikeda, Kazutaka; Kita, Yoshihiro; Horie, Kanta; Saigusa, Daisuke; Saito, Kosuke; Sawada, Yuji; Nakanishi, Hiroki; Okahashi, Nobuyuki; Takahashi, Masatomo; Nakao, Motonao; Hata, Kosuke; Hoshi, Yutaro; Morihara, Motohiko; Tanabe, Kazuhiro; Bamba, Takeshi; Oda, Yoshiya.

:: Metabolites, 巻 9, 番号 11, 257, 11.2019.

研究成果: ジャーナルへの寄稿記事

Izumi, Y, Matsuda, F, Hirayama, A, Ikeda, K, Kita, Y, Horie, K, Saigusa, D, Saito, K, Sawada, Y, Nakanishi, H, Okahashi, N, Takahashi, M, Nakao, M, Hata, K, Hoshi, Y, Morihara, M, Tanabe, K, Bamba, T & Oda, Y 2019, 'Inter-laboratory comparison of metabolite measurements for metabolomics data integration', Metabolites, 巻. 9, 番号 11, 257. https://doi.org/10.3390/metabo9110257
Izumi, Yoshihiro ; Matsuda, Fumio ; Hirayama, Akiyoshi ; Ikeda, Kazutaka ; Kita, Yoshihiro ; Horie, Kanta ; Saigusa, Daisuke ; Saito, Kosuke ; Sawada, Yuji ; Nakanishi, Hiroki ; Okahashi, Nobuyuki ; Takahashi, Masatomo ; Nakao, Motonao ; Hata, Kosuke ; Hoshi, Yutaro ; Morihara, Motohiko ; Tanabe, Kazuhiro ; Bamba, Takeshi ; Oda, Yoshiya. / Inter-laboratory comparison of metabolite measurements for metabolomics data integration. :: Metabolites. 2019 ; 巻 9, 番号 11.
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AU - Hirayama, Akiyoshi

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AU - Kita, Yoshihiro

AU - Horie, Kanta

AU - Saigusa, Daisuke

AU - Saito, Kosuke

AU - Sawada, Yuji

AU - Nakanishi, Hiroki

AU - Okahashi, Nobuyuki

AU - Takahashi, Masatomo

AU - Nakao, Motonao

AU - Hata, Kosuke

AU - Hoshi, Yutaro

AU - Morihara, Motohiko

AU - Tanabe, Kazuhiro

AU - Bamba, Takeshi

AU - Oda, Yoshiya

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